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- Using a 60 MHz spectrometer, a chemist observes the following peak: doublet, J = 7 Hz, at 4.00 ppm How many hertz away from the TMS (tetramethylsilane, defined as 0 ppm) peak would this peak be in a spectrum collected on a 300 MHz spectrometer?The intensities of peaks at 69 m/z, 70 m/z, and 71 m/z are 1.2, 31.2, and 1.7, respectively. How many carbons does the compound have?(a) What would be the chemical shift of a peak that is observed at 655.2 Hz from the reference tetramethylsilane (TMS) recorded using a 90 MHz spectrometer ? (b) At what frequency would the chemical shift of chloroform (CHCl3, δ = 7.28 ppm) occur relative to TMS on a spectrum recorded on a 300 MHz spectrometer? (c) At what frequency and chemical shift would the signal for chloroform occur when using a 1 GHz NMR spectrometer?
- Using a 60-MHz spectrometer, a chemist observes the following absorption:doublet, J = 7 Hz, at d 4.00(a) What would the chemical shift (d) be in the 300-MHz spectrum?(b) What would the splitting value J be in the 300-MHz spectrum?(c) How many hertz from the TMS peak is this absorption in the 60-MHz spectrum? In the 300-MHz spectrum?Using a 60 MHz spectrometer, a chemist observes the following peak: doublet, J = 7 Hz, at 4.00 ppm How many hertz away from the TMS (tetramethylsilane, defined as 0 ppm) peak is this peak in the 60 MHz spectrum?molecular formula: C5H9N 1. Given the molecular formula, label important peaks on the spectrum and explain how you determined the Structural Formula for your assigned compound.
- The molecule β-carotene has λmax 450 nm, and ɛ = 15,000 m2 mol-1. Calculate the absorption (A) expected for a solution in which 0.1 mg has been dissolved in 10 ml of water (given: the molecular weight of β-carotene, C40H56, as 536) with a path length of 1 cm. 2.8 0.28 2.8 x 10-4 0.028The M+ peak is at 73 m/z. What atom besides carbon and hydrogen is present in the compound, and what is the compound’s chemical formula? What is the structure for the M+ peak and the structures for the peaks at 58 and 44 m/zThe 1H NMR spectrum of 1,2-dimethoxyethane (CH3OCH2CH2OCH3) recorded on a 300 MHz NMR spectrometer consists of signals at 1017 Hz and 1065 Hz downfield from TMS.(a) Calculate the chemical shift of each absorption. (b) At what frequency would each absorption occur if the spectrum were recorded on a 500 MHz NMR spectrometer?
- A researcher is studying cytochrome oxidase, a protein that uses iron to bind diatomic oxygen. She collects two IR spectra, shown below. In the first spectrum, no isotopic labeling is used (top). In the second, 10 has been replaced with 180 (bottom). 820 655 790 0.5- 714 695 733 820 655 755 0.5- 714 695 733 0+ 600 650 700 750 800 850 Vo (cm") Based on the above ratio, which is the most likely source of this vibration, (a) the Fe-O stretch in Fel-O=0, or (b) the O-O stretch in Fel"-O=O? You can assume that the atomic weight of Fe is 56. ww n wwm % TransmissionA researcher is studying cytochrome oxidase, a protein that uses iron to bind diatomic oxygen. She collects two IR spectra, shown below. In the first spectrum, no isotopic labeling is used (top). In the second, 160 has been replaced with 180 (bottom). 820 655 790 0.5- 714 695 733 820 655 755 0.5- 714 695 733 0- 600 650 700 750 800 850 Vo (cm") Based on the above ratio, which is the most likely source of this vibration, (a) the Fe-O stretch in Fell-O=0, or (b) the O-O stretch in Fell-O=O? You can assume that the atomic weight of Fe is 56. % Transmission3. Find the position of the absorption peak of a low-resolution spectrum of 12C180