3. Now for the citric acid cycle, it is time to follow the carbons! Using labeled pyruvate: 14CH3¹3COCOO- trace the fate of that pyruvate 'when added to respiring mitochondria. a. After one turn of the citric acid cycle what is the location of each labeled carbon? Show this by drawing the structural formulas for the TCA intermediates. b. What is the consequence of the metabolism of succinate with respect to the labeled atoms? Cellular respiration can be studied in isolated mitochondria by measuring oxygen consumption under different conditions. If 0.01 M sodium malonate is added to actively respiring mitochondria that are using pyruvate as fuel source, respiration soon stops, and a metabolic intermediate accumulates. What is the name and structure of this intermediate? Why does it accumulate? What is sodium malonate doing and how? C. d. ( e. I Explain. Aside from removal of the malonate, how can this inhibition of respiration be overcome?
3. Now for the citric acid cycle, it is time to follow the carbons! Using labeled pyruvate: 14CH3¹3COCOO- trace the fate of that pyruvate 'when added to respiring mitochondria. a. After one turn of the citric acid cycle what is the location of each labeled carbon? Show this by drawing the structural formulas for the TCA intermediates. b. What is the consequence of the metabolism of succinate with respect to the labeled atoms? Cellular respiration can be studied in isolated mitochondria by measuring oxygen consumption under different conditions. If 0.01 M sodium malonate is added to actively respiring mitochondria that are using pyruvate as fuel source, respiration soon stops, and a metabolic intermediate accumulates. What is the name and structure of this intermediate? Why does it accumulate? What is sodium malonate doing and how? C. d. ( e. I Explain. Aside from removal of the malonate, how can this inhibition of respiration be overcome?
Biochemistry
6th Edition
ISBN:9781305577206
Author:Reginald H. Garrett, Charles M. Grisham
Publisher:Reginald H. Garrett, Charles M. Grisham
Chapter27: Metabolic Integration And Organ Specialization
Section: Chapter Questions
Problem 20P: Figure 27.3 illustrates the response of R (ATP-regenerating) and U (ATP-utilizing) enzymes to energy...
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