25 nm, which çan be used for the spectrophotometric determination of ammonia. OCF 2 OH + NH3 indophenol anion To determine the ammonia concentration in a sample of lake water, three samples are prepared. In sample A, 10.0 mL of lal water is mixed with 5 mL of phenol solution and 2 mL of sodium hypochlorite solution, and diluted to 25.0 mL in a volume lask. In sample B, 10.0 mL of lake water is mixed with 5 mL of phenol solution, 2 mL of sodium hypochlorite solution, and 2.50 mL of a 5.50 x 10-4 M ammonia solution, and diluted to 25.0 mL. Sample C is a reagent blank. It contains 10.0 mL of distilled water, 5 mL of phenol solution, and 2 mL of sodium hypochlorite solution, diluted to 25.0 mL. The absorbance of t three samples is then measured at 625 nm in a 1.00 cm cuvette. The results are shown in the table. Sample Absorbance (625 nm) A 0.361 В 0.608 C 0.045 What is the molar absorptivity (e) of the indophenol product? E = M-'cr

Principles of Instrumental Analysis
7th Edition
ISBN:9781305577213
Author:Douglas A. Skoog, F. James Holler, Stanley R. Crouch
Publisher:Douglas A. Skoog, F. James Holler, Stanley R. Crouch
Chapter9: Atomic Absorption And Atomic Fluorescence Spectrometry
Section: Chapter Questions
Problem 9.19QAP
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Treatment of ammonia with phenol in the presence of hypochlorite yields indophenol, a blue product that absrobs light at
625 nm, which çan be used for the spectrophotometric determination of ammonia.
gan
OCF
OH + NH3
indophenol anion
To determinè the ammonia concentration in a sample of lake water, three samples are prepared. In sample A, 10.0 mL of lake
water is mixed with 5 mL of phenol solution and 2 mL of sodium hypochlorite solution, and diluted to 25.0 mL in a volumetric
flask. In sample B, 10.0 mL of lake water is mixed with 5 mL of phenol solution, 2 mL of sodium hypochlorite solution, and
2.50 mL of a 5.50 x 10-4 M ammonia solution, and diluted to 25.0 mL. Sample C is a reagent blank. It contains 10.0 mL of
distilled water, 5 mL of phenol solution, and 2 mL of sodium hypochlorite solution, diluted to 25.0 mL. The absorbance of the
three samples is then measured at 625 nm in a 1.00 cm cuvette. The results are shown in the table.
Sample Absorbance (625 nm)
A
0.361
B
0.608
C
0.045
What is the molar absorptivity (e) of the indophenol product?
E =
M-'cm-!
What is the concentration of ammonia in the lake water?
(NH,ljake water
M
Transcribed Image Text:Treatment of ammonia with phenol in the presence of hypochlorite yields indophenol, a blue product that absrobs light at 625 nm, which çan be used for the spectrophotometric determination of ammonia. gan OCF OH + NH3 indophenol anion To determinè the ammonia concentration in a sample of lake water, three samples are prepared. In sample A, 10.0 mL of lake water is mixed with 5 mL of phenol solution and 2 mL of sodium hypochlorite solution, and diluted to 25.0 mL in a volumetric flask. In sample B, 10.0 mL of lake water is mixed with 5 mL of phenol solution, 2 mL of sodium hypochlorite solution, and 2.50 mL of a 5.50 x 10-4 M ammonia solution, and diluted to 25.0 mL. Sample C is a reagent blank. It contains 10.0 mL of distilled water, 5 mL of phenol solution, and 2 mL of sodium hypochlorite solution, diluted to 25.0 mL. The absorbance of the three samples is then measured at 625 nm in a 1.00 cm cuvette. The results are shown in the table. Sample Absorbance (625 nm) A 0.361 B 0.608 C 0.045 What is the molar absorptivity (e) of the indophenol product? E = M-'cm-! What is the concentration of ammonia in the lake water? (NH,ljake water M
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