1. Provide a reasonable step-wise mechanism for the reaction below, involving FAD as a coenzyme and invoking any general acids and bases from acyl-CoA dehydrogenase that you might need (just draw the tricyclic flavin core of FAD; no need to show the ribitol or ADP, R + FAD SCOA acyl-CoA dehydrogenase R i SCOA + FADH2
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Please draw the reaction mechanism and include arrows .
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- Extending the Mechanism of Methylmalonyl-CoA Mutase to Similar Reactions Based on the mechanism for the methylmalonyl-CoA mutase (see problem 14), write reasonable mechanisms for the following reactions shown.Understanding the Mechanism of the HMC-CoA Synthase Reaction Write a suitable mechanism for the HMG-CoA synthase reaction. What is the chemistry that drives this condensation reaction?Arrange the steps of breakdown of oleic acid (pictured below) in the correct order. 1 2 3 4 5 activation by acyl-CoAsynthe ✓ [Choose ] one cycle ofß-oxidationbeginning at the enoyl-CoAhydratasestep three cycles ofẞ-oxidation five cycles ofß-oxidation activation by acyl-CoAsynthetase enoyl-CoAisomeraseactivity enoyl-CoAisomeraseactivity V one cycle ofß-oxidationbegini V five cycles ofß-oxidation OH
- Provide a reasonable step-wise mechanism for the reaction below, involving FAD as a coenzyme and invoking any general acids and bases from acyl-CoA dehydrogenase that you might need (just draw the tricyclic flavin core of FAD; no need to show the ribitol or ADPasap8. The enzyme thiolase catalyzes one step in the ß-oxidation of saturated fats. One portion of the mechanism for this reaction is shown below. Describe the catalytic mechanism at work. (Note: "SCOA" is shorthand for the compound Acetyl-CoA) RCOCH₂COSCoA + HSCOA → H3C₂OSC0A + RCOSCOA HN NH CH₂ NH CH₂ CH₂ FS Grease NH3 H-SCO A NH CH2 CH₂ NH3 S Co A CH₂ NH 1 3 H-SCO A H3C SCO A NH3 a h-SCOA R-C S Co A CH₂ CH₂ C H₂ 0=0 HN NH + NH3 H₂C HN CH₂ NH CH₂ CH₂ 2 S Co A CH₂ NH NH
- 4. Identify: for nos. 1-5: Name of the missing metabolite in the pathway 6-10: Enzyme that catalyzed the reaction 11-15: Reaction involved 5 FAD 10 -FADH₂ trans-2-En oyl-CoA 15 H₂O 3-Hyroxya cyl-CoA NAD+ NADH+H+ beta-Ketoacyl-CoA COASH Acetyl-CoA Acyl-CoA (2 C Atoms Shorter) Malate Fumarate Glucose 6 Glucose-6-phosphate 11 Fructose-6-phosphate Phoshphoenolpyruvate Succinate 12 Acetyl ČOA 13 7 Citrate 3 Lactate 14 Isocitrate 8 a-ketoglutarate1. Consider the reaction: succinyl-CoA succinate H3C C H₂ a. What kind of reaction is being performed here? H³C C H₂ b. What enzyme performs this reaction? S-COA c. What cofactors, if any, are required for this reaction?2. Draw the reactions catabolizing the amino acid valine into isobutryl-CoA, specifically one transamination reaction (for which you do not need to show the mechanism) and one dehydrogenation reaction (which is analogous to the pyruvate dehydrogenase reaction mechanism, and for which you need to show the mechanism using arrow pushing and including the relevant cofactors). Note: Beyond the importance of these reactions for human health, this pathway is used industrially in the production of isobutanol, an important biofuel. NH3 SCOA valine isobutyryl-CoA
- 19. Which of the following statements is true for the shown reactions? i C-S-COA CH₂ H₂C-C-OH CH₂ coo W Coo CH₂ C=0 I Acetyl-CoA CH3 x 214 Coo- 1 CH ₂ I CHOH CH3 Y A. Deficiency of oxaloacetate stimulates the formation of X and y B. Insulin signaling stimulates the formation of X and Y C. Both A and B D. Neither A nor B33. Which of the following statements is true for the shown reaction? P-O-CH₂ P-O-CH₂ OH A Coo I C=O HOTHS CH₂ Coo A HỌ он D A. The reaction is catalyzed by PFK-1 B. Stimulation of the reaction eventually slows down gluconeogenesis C. Inhibition of glyceraldehyde 3-phopshate dehydrogenase leads to the accumulation of the product B D. All of the above E. None of the above OH 34. Which of the following statements is true for the shown reaction? COO™ CH₂OH HQ -℗ B CH₂ B C-o-℗ + CO₂ A. Insulin can eventually inhibit the reaction B. The reaction only take place in the presence of acetyl-CoA C. The product B is a high-energy molecule, and the next logical step is to use the energy released from B to synthesize ATP D. All of above E. None of the aboveConsider the complete oxidation of one mole of the fatty acyl CoA of myristic acid (14:0). Determine the following (Type the numerical value only - e.g. 1): 1. rounds of B-oxidation 2. total no. of acetyl CoA produced 3. total no. of NADH produced from all rounds of B-oxidation 4. total no. of FADH2 produced from all rounds of B-oxidation 5. total no. of NADH produced from the oxidation of all acetyl CoA in the TCA cycle 6. total no. of FADH, produced from the oxidation of all acetyl CoA in the TCA cycle 7. total ATP yield