1. Ifa Lineweaver-Burk plot gave a line with an equation of y=0.25 x +0.34, what are the values of KM and Vmax if the substrate concentration is in mM and the velocity in mM/s?
Q: Individuals with a thiamine-deficient diet have relatively high levels of pyruvate in their blood.…
A: The diseases that occur because of insufficient intake of essential nutrients are called deficiency…
Q: A contributing factor to the development of arthritis is the inappropriate proteolytic destruction…
A: An assay is a biochemical lab procedure to determine the quantity of molecules of interest in a…
Q: The dominant motif found in hemoglobin and myoglobin is: a) twisted beta sheet b) beta barrel c)…
A: 1)The dominant motif found in hemoglobin and myoglobin is (b) beta barrel.2)myoglobin consists of (d…
Q: 1. Start with the Michaelis-Menten equation and convert it to a double-reciprocal equation. Show how…
A: For a one-substrate enzyme-catalyzed reaction, the Michaelis-Menton equation shows the quantitative…
Q: Which of the following are characteristics of isoprenoid lipids? double bonds are mostly cis. methyl…
A: A type of organic molecules known as isoprenoid lipids is made up of two or more hydrocarbon units,…
Q: Look at the synthesis of citryl CoA by citrate synthase in the figure. Which of the 4 general…
A: Citrate synthase is an important enzyme in citric acid cycle that catalyses the conversion of oxalo…
Q: what are the non-regulatory enzymes involved in glycolysis.
A: Glycolysis is the 10-step enzymatic oxidation of a single glucose molecule into two molecules of…
Q: 6. ANSWER THE FOLLOWING PROBLEM AND EXPLAIN YOUR ANSWER FOR BETTER UNDERSTANDING. Fill in the blanks…
A: There are four classes of biological macromolecules: They are proteins, nucleic acids, lipids and…
Q: gluscose trasporter in teh liver cell binds with Km of 40mM. Calculate what fraction of vmax the Vo…
A: Km is the kinetic constant that determines the affinity of an enzyme to its substrate.Vmax is…
Q: Consider a small protein containing 98 amino acid residues. The protein backbone will have 194 bonds…
A: Assuming that each of the 194 rotatable links in the protein backbone can have three alternative…
Q: Provide an overview of the components and structure of a microchip.
A: The scientific field of biochemistry studies the compounds and chemical reactions that take place…
Q: Out of starch, glycogen, cellulose and chitin, carbohydrate polymers with beta linkages are O…
A: The alpha and beta linkages are types of glycosidic linkages which are formed between the hemiacetal…
Q: what is the relation between K'eq and mass action ratio (Q) for biochem? specifically what are the…
A: Chemical reactions are favourable when they result in a decrease in enthalpy and an increase in…
Q: It is important for a protease to distinguish between two different proteins so that it only cleaves…
A: The building blocks of proteins are organic substances called amino acids. They have a side chain (R…
Q: Lipids in a bilayer can diffuse laterally at a relatively fast rate, but "flip-flop" from one…
A: Flippases, floppases, and scramblases are phospholipid translocators present in the membrane…
Q: Consider the structure of calmodulin. Identify the correct statements about calmodulin. Calmodulin…
A: Calmodulin is a Ca2+ binding protein. It associates with many other proteins and thereby regulates…
Q: Which of the following stretches of RNA is most likely to form a hairpin with 4 bases making up the…
A: A nucleotide (pentose sugar + nitrogenous base + phosphate group) is a phosphoric acid ester of a…
Q: Rank the following fatty acids from lowest melting temperature to highest melting temperature. (A)…
A: Fatty acids are carboxylic acids with a hydrocarbon chain ranging from 4 carbons to 36 carbons. The…
Q: 2. An enzyme-catalyzed reaction has a Km = 120 μM. At what substrate concentration would this…
A: In enzyme kinetics, the Michaelis-Menten constant, or Km, is a parameter that characterises the…
Q: The steps involved in the T→ R transition in when oxygen binds to hemoglobin are outlined below.…
A: The correct order of the steps involved in the T → R transition in hemoglobin when oxygen binds is…
Q: 1.) The protein SIRT6 has been shown to be important in energy metabolism and inflammatory…
A: Enzyme are proteins that catalyse biochemical reactions. An enzyme's activity refers to the rate at…
Q: The scientific name of the fructose molecule shown below is CH2OH OH НО ОН CH₂OH
A: Fructofuranose is a structure which is similar to the furan ring, which has a five carbon membered…
Q: Suppose a researcher previously cloned gene Y into M13 bacteriophage vector. Gene Y encodes a…
A: In order to solve this problem, first we need to find the amino acid sequence of the wild (original)…
Q: The effect of an inhibitor on an enzyme was tested and the experiment gave the results below. If…
A: Inhibition can be either reversible or irreversible. Reversible inhibition can be generally of 3…
Q: 4. Draw a chemical mechanism (with electron-pushing arrows) for how glycine is added to a…
A: Ribosomes are protein-RNA complexes that catalyse peptide bond formation. A peptide bond is formed…
Q: A mutation of Lysine to Alanine in the middle of TM1 has been associated with increased GPCR…
A: The lipid bilayer is a thin polar membrane. It is made up of two layers of lipid molecules. The…
Q: How many different reactions (enzymes) in the glycolytic pathway (steps 1 - 10) involve ATP either…
A: In glycolysis, a 6-carbon molecule of glucose-6-phosphate is broken down into 2 molecules of…
Q: How does Foldit Protein Folding Game use puzzles like Design, De Novo, Revisiting, help in…
A: The Department of Biochemistry and the Center for Game Science at the University of Washington…
Q: Calculate the membrane potential in the transport of Cl- from the intracellular environment to the…
A: Change in Gibbs free energy () for ion transport across a membrane is contributed by the…
Q: 6 9 ation Which of the following is an unacceptable structure of a monosaccharide sugar? А. C. HOCH2…
A: Monosaccharides are the most simple sugars/carbohydrates. These are composed of carbon, hydrogen and…
Q: Can you explain the various options and how did you come to the answer? How to know whether histine…
A: The proteins are constituted of 20 naturally occurring amino acids.Histidine is an essential amino…
Q: In considering active transport by Na + -K + -ATPase at body temperature (37 o C), 3 Na+ are pumped…
A: The following equation describes the mathematical relation for the change in free energy required…
Q: Why must NH3 be transported as part of an amino acids vs. being released into circulation? What…
A: Ammonia is produced in all tissues of the body during the metabolism of amino acids and other…
Q: Model 1 Model 1 Idealized Graphs of Enzyme inhibition Competitive Inhibition FEE Inh. slope How does…
A: Since you have posted multiple questions, we will provide the solution only to the first question as…
Q: Identify the species that are liganded to the porphyrin ring Fe(II) ion in deoxyhemoglobin (i.e., Hb…
A: The protein haemoglobin, which is present in red blood cells, is essential for the movement of…
Q: A contributing factor to the development of arthritis is the inappropriate proteolytic destruction…
A: A variable is any quantity we can measure. In any experiment there are three variables: Independent:…
Q: Calculate AG' at 25 °C for the reaction: ATP + pyruvate = ADP + phosphoenolpyruvate Calculate Keq at…
A: The change in Gibbs free energy (ΔG) is the maximum amount of free energy available to do useful…
Q: Given the following peptide SEPLMAPVEYPK TABLE 5.1 Properties of the common amino acids found in…
A: pKa is the pH at which the weak acid is 50% dissociated.When pH < pKa of the ionising group, the…
Q: Which of the following amino acids are aromatic in nature? Select the correct response(s): F [1 H K…
A: The building blocks of proteins are amino acids. They have three groups: an amino group, a carboxyl…
Q: Which of the equations below best represents how excess acid in the blood plasma can be removed in…
A: All biological processes are pH dependent. Even a slight change in pH can result in a large change…
Q: Write out the mechanism for transimination, the reaction of an amino acid with enzyme-bound PLP to…
A: The transimination reaction, also known as the Schiff base formation,involving the transfer of a…
Q: Describe the structures of glutamine and alanine. What are the characteristics of the R-groups?…
A: There are four types of biological macromolecules: nucleic acid, proteins, lipids and…
Q: Draw a representation of an allosteric binding site of an enzyme. The allosteric site must bind an…
A:
Q: (e) [S] = 10KM 051000091 ods 26. MATHEMATICAL Determine the values of Kỵ and Vmax for the de-…
A: Km and Vmax are kinetic constants that help to determine the efficiency of an enzyme.Km is equal to…
Q: Suppose you visit the Dalai Lama in Dharamsala, India (elevation 1460 m), and you begin to ponder…
A: Fractional Saturation (θ) is the fraction of protein molecules saturated with ligands. (since it is…
Q: Draw the skeletal ("line") structure of an aldehyde with 3 carbons in the main chain and 1 methyl…
A:
Q: Which of the processes are coupled to the dephosphorylation of ATP? membrane transport that…
A: The structure of ATP comprises of a nucleoside triphosphate, consisting of a nitrogenous base…
Q: Classify the phrases. Does each phrase describe a kinase, a phosphatase, neither, or both? Kinases…
A: Proteins are regulated by adding or removing phosphate groups. These are crucial for the…
Q: Which of the following runs an identical application as the production environment? Select one: Oa.…
A: The natural world that hosts living things is known as the environment. It consists of the…
Q: The F₁F. - ATP synthase is a molecular machine that converts the proton motive force into enzymatic…
A: The molecular device known as F1F0-ATP synthase transforms the proton motive force into enzymatic…
Trending now
This is a popular solution!
Step by step
Solved in 3 steps
- Plot a line weaver-burk graph for Km=6.30mM and Vmax=360uM/min when an experiment has 5 tubes with concentrations of substrates of 1.0mM, 10mM, 50mM, and 100mM.From the equation of a lineweaver-burke plot, calculate Km and Vmax. Show your work and include units. The equation is y=393.4x +8.4337The equation of the double reciprocal plot is y = 0.5294 x + 1.4960. What is the value of vmax (in M/s)? The substrate concentration is given in units of molarity (M) and reaction velocity has units of molarity per second (M/s). (Report to three significant figures)
- Consider the titration of 50.00 mL of 0.160 M NH3 with 0.200 M HCl. Calculate the pH at the following volumes of the titrant: VT = 0.00 (Initial Stage) VT = 20.00 VT = 30.00 VT = 40.00 (Equivalence Point Stage) VT = 45.00 Kb = 1.76 x 10-5You obtained the following raw data when setting up a Biuret standard curve: Absorbancy BSA (mg/ml) 540nm 0.158 1 0.210 0.260 0.305 0.360 0.410 0.455 7 0.510 0.530 0.550 10 0.554 What would the quality of the line-fit (R2 value) be if you do not exclude experimental outliers? (Give you answer to 4 decimal places) O-2N 34 56 o9SUBSTRATE CONCENTRATION [S] µM INITIAL VELOCITY V0 s-1 10 0.13 25 0.27 50 0.45 100 0.65 150 0.77 200 0.85 300 0.94 500 1.03 (i) a) Construct an empty table with the following column headings: Substrate concentration [S] and initial velocity (Vi) where [S] has the unit µM, and Vi has the unit mM/s. (ii) The table provided is the enzyme kinetic data for your mutated enzyme, whereby Vi was expressed using the unit ∆A(405 nm)/s. Using the standard curve, express Vi with the unit mM/s rather than ∆A(405 nm)/s. Place your answer in the table above alongside the appropriate [S]. Hint: To answer this question you need to use this standard curve equation=0.0419x (The slope of the line is= 0.0419) (iii) The unmutated form of your protein has a Km of 25 µM and a Vmax of 43 mM/s. The enzyme kinetic data for your enzyme with the amino acid substitution should now be displayed in the table above. Based on these data, what is Vmax? Km? and…
- An enzyme with concentration of 3.0 uM is added to a flask containing 125 uM of substrate. You know the Km is equal to 0.054 mM, and has a Vmax of 1.4 uM/s. What is the observed velocity? O 0.98 uM/s 0.074 uM/s O 5.2 um/s 1.4 uM/sTwo compounds with partition coefficients of 15 and 18 areto be separated on a column with Vm/Vs 3.0 and tm 1.0 min.Calculate the number of theoretical plates needed to produce aresolution of 1.5.Write C if only statement A is correct, H if only statement B is correct, E if both statements are correct, M if both statements are incorrect. (A) For the Lineweaver-Burk plot, the slope of the line reflects the ratio between the maximum velocity and the Michaelis constant. (B) Meanwhile, the y-intercept is the maximum velocity.