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1. Define and Describe aseptic technique in blood collection.
2. Explain the action of SPS as anticoagulant for blood culture.
Step by step
Solved in 2 steps
- 1. cite the differences between tube dilution and microtiter plate dilution. 2. why is it important to isolate the peripheral blood mononuclear cells completely from the other cellular components of the blood? 3. what laboratory assays can be performed to assess the efficiency of the sample preparation and isolation of the PBMC?3. Explain the purpose of thixotropic gel in a blood collection tube.&9. Explain the principle and step by step procedure of Dry Blood Spots collection.?
- 1. How will you differentiate VDRL from RPR Card test? 2. Give the composition of VDRL reagent and their function.1. Why is it necessary to avoid underfilled blood samples in anticoagulant evacuated tubes? 2. Explain the purpose of thixotropic gel in a blood collection tube. 3. What is the rationale behind the sequence of the order of draw? a. Sterile tube (blood culture) b. Blue-top coagulation tube C. Serum tube (glass) with no additive d. Serum tube with clot activator, with or without е. Heparin tube with or without gel plasma separator f. EDTA tube g. Glycolytic inhibitor tube 4. What are the potentially affected tests if an evacuate tube with silica clot activator was filled first before a blue-top coagulation tube?Explain the action of SPS as anticoagulant for blood culture
- 1.What is the purpose of priming?2. Typically how much volume (ml) of saline is needed for the priming?1. Differentiate the 2 specific types of VDRL namely: 1.1 quantitative VDRL 1.2 qualitative VDRL 2. Describe the principle behind the RPR (Rapid Plasma Reagin) Test. 3. What precautionary measures should be observed in the collection and preparation of specimen for VDRL examination?2. Describe the collection of dry blood spots' basic principles and step-by-step methodology.
- 1. Give 2 example of methods used for fresh tissue examination. Explain each method. 2. Enumerate and explain briefly all the types of smear preparation.1. define the latex agglutination (LA) and gelatin particle agglutination tests (GPAT) techniques 2. State the principle underlying the above techniques 3. state the types or forms of the techniques if present1. What happens if sample for Potassium determination is refrigerated? What causes the variation? 2. What is the role/importance of Potassium in the body metabolism? 3. In doing the assay, why is hemolyzed sample not allowed?