Laboratory 2: Scientific Methodology & Enzyme Activity Objective: The purpose of this experiment was to simply measure oxygen production rates released from decomposed hydrogen peroxide under different conditions (concentration of enzymes, temperature, and PH level). Hypothesis: Part a: If different amounts of enzyme solution are added to the hydrogen peroxide, then the highest amount of enzymes will have the greatest reaction rate because enzymes catalyze reactions, meaning more oxygen will be produced quicker. Part b: If different temperatures are used to catalyze enzyme activity, then the lowest temperature would produce the quickest reaction rate because enzymes can become denaturized at higher temperatures …show more content…
Part b: These results show how temperature of extreme high, or low affects enzyme activity. The highest rate of enzyme activity occurred at 37 Cº. Anything that was hotter or cold than 37 Cº slowed the reaction rate. As I thought, 100 degrees would denature the enzyme, and that was the case. The data provided shows exactly what temperatures enzymes work best, and worst. The objective was achieved as we discovered the different reaction rates under different temperatures. The results are reliable, as we know enzymes do not work well when under extreme heat or denaturation occurs. What I learned in this experiment was that enzymes don’t work well under cold temperatures because they tend to move slower. My hypothesis did not quite match, because I thought they work best at lower temperatures. Part c: These results shown from this experiment led us to conclude that enzymes work best at certain pH rates. For this particular enzyme, pH 7 worked best. When compared to high levels of pH, the lower levels worked better. The wrong level of pH can denature enzymes; therefore finding the right level is essential. The independent variable was the amount of pH, and the dependent being the rate of oxygen. The results are reliable as they are reinforced by the fact that enzymes typically work best at neutral pH
The more acidic a substance is the less oxygen it will produce when going through a chemical reaction. During the Lab “How Do Changes in pH Levels Affect Enzymes Activity”, the researcher conducted an experiment to test the effects that an acidic, neutral, and a base substance will have when combine it with hydrogen peroxide. The data table shows that HCL (acidic substance) barley produced any oxygen at all when it was combining with Hydrogen Peroxide. The pH level for HCL was 2.5; this level indicates that the substance was very acidic. When the H2O and NaOH were tested they produced more bubbles than HCL. NaoH produced a little more bubbles than HCL. The pH that NaoH produced was a 9, which is a base. H2O produced more bubbles than both substances;
Most chemical reactions speed up as temperature is raised. As the temperature is raised, kinetic energy within the molecules increases as well to endure the reaction. Because enzymes are catalysts, meaning they make a chemical reaction react faster, enzyme reactions also tend to go faster with increase temperature. However, if the temperature of an enzyme-catalyze is raised to a temperature optimum, the kinetic energy in the water molecules and the enzyme becomes too high and the enzyme starts to denature, meaning the molecules become disputed. Most enzymes are denatured by around 104 degrees Fahrenheit to about 122 degrees Fahrenheit. Denaturing is disabling an enzyme by changing the normal qualities or the nature of it, for instance when
The temperature can have a major impact on an enzyme. According to Campbell Biology author Reece etc. 2011 “The enzyme reaction will increase as the temperature increase with the increasing temperature….substrates collide with active sites more frequently when the molecules move rapidly.”(Reece etc 2011) Every enzyme hits its optimal temperature the reaction will be at its highest point.(Reece etc. 2011) When the
Temperature controls the speed the enzymes work at. Higher temperatures increase the kinetic energy which increases the chance of collision therefore speeding up the rate of
The purpose of this lab was to study the effect of varying the substrate concentration of Hydrogen peroxide (H2O2) on the rate of peeled potato catalase enzyme activity, on the decomposition of H2O2. This experiment was carried out by diluting the 6% H2O2 with distilled water to decrease the concentration, and then placed in a test tube. A small piece of filler paper disc was coated with the enzyme before it was submerged to the bottom of the substrate hydrogen peroxide solution. The stop timer was started immediately when the filter paper disc touched the bottom and the timer was stopped when the filter paper reached the surface. The results of the experiment showed a linear correlation between concentration of the substrate and the rate of enzyme activity. Overall, as the concentration of H2O2 was decreased, the rate of reaction had decreased.
As previously stated, temperature is another variable that can affect the enzyme reaction rate. At low temperatures there will be little to no activity but as the temperature is increased so the rate of activity will increase. However, just like pH, once the optimal temperature is reached and surpassed, the enzyme will begin to break apart. The enzyme will be experimentally tested using temperatures around room temperature (27℃). Due to the fact that there is a human application the enzyme will continue to be tested until temperatures of human death (45℃). After the testing is completed it can be deduced what temperatures create an optimum environment for the normal functioning of the enzyme. One
This data supported this showing an increase in the rate of reaction as the temperature got closer to the optimum point of temperature, which in this case was 20 C degrees. However, this did not support the hypothesis that the rate of reaction would increase as the temperature got closer to 37 C degrees. The data after the solutions in the tubes had all been brought back to 37 C degrees showed that those tubes in low temperature started to have an increase in rate of reaction. This is because the effects of low temperature are reversible. However, the data showed that the denaturizing at high temperatures is not reversible, the test tube heated to 70 C degrees had a slower rate of reaction when the temperature was brought to 37 C degrees. The reason enzymes have this optimum point is because the thermal agitation effect the bonds found in the enzyme causing it to lose its structure. Without this structure the enzyme can no longer catalyze the reactions. This goes both ways, but where the enzymes bonds are disrupted at high temperatures, the rate of reaction is slowed at low temperatures because the molecules move at a slower general
It is predicted that enzyme activity will speed up from 0 to 37 degrees and then rapidly slow down or stop altogether at 55 degrees. Methods For this experiment the dependent variable will be the amount of product, oxygen, produced by the enzyme catalase breaking down hydrogen peroxide. The independent variable will be temperature. Four temperatures will be tested on the enzyme to see how much product will be produced. Controlled variables will be the amount of enzyme, hydrogen peroxide and water used in the test tubes, as well as the volume of the nalgene bottle (250 mL) used in testing to measure the product.
During the lab various factors were changed in order to compare how different environmental changes affected the rate of the reaction. A first test was conducted with liver and hydrogen peroxide without an induced temperature change scored a 5. Next, the catalase (liver) was heated to a high temperature and then hydrogen peroxide was added. The reaction was not as fast, and produced a score of 3. A similar test was done using another piece of liver but was placed in an ice bath and then hydrogen peroxide was added. This produced an even slower reaction rate of 2. These three tests demonstrate how temperature greatly influences the rate of enzyme action. When enzymes reach above boiling point, they are denatured and no longer function. Optimal temperatures for enzymes to function is 35-40 degrees Celsius. When the temperature is lower than optimal, slow reactions occur. Further experiments were conducted to show how pH levels affected enzyme action. Two mL of hydrogen peroxide was added to three test tubes. Then, HCl was added to test tube 1, NaOH was
THE EFFECT OF TEMPERATURE ON THE RATE OF ENZYME ACTIVITY DB-16-0081 Co-partner: TIMORE NURDINOV Introduction The aim from this experiment is to observe the effect of factors such as pH, temperature and inhibitor on enzyme activity and how it works.
Enzymes are proteins that functions as a catalyst in chemical reactions that occur in living organisms. The rate of enzyme activity can be effected by many factors, including pH, temperature, enzyme concentration and substrate concentration. This experiment will be focusing on the first three factors. Enzymes are sensitive and can only work under optimum conditions in order to function properly. If conditions are not optimum, the rate of enzyme activity will decrease, or the enzyme could become
Investigating Enzyme Activity Aim: To investigate how the concentration of hydrogen peroxide effects the rate of reaction of an enzyme (catalase) Variables: These factors could effect the rate of reaction on an enzyme: · pH · Concentration · Temperature · Surface Area pH - Enzymes function at different pH values. In neutral conditions the amount of oxygen gas given of in an enzyme-catalysed reaction will increase. An enzyme is affected by how much acid or alkali is present. Many enzymes work best in neutral conditions but some prefer acids and some prefer alkalis.
In this experiment we observed a specific enzyme-catalyzed reaction and designed two experiments in order to test the effects of different factors on enzyme activity. The specific reaction given was 2H2O2=2H2O + O2. For our resolutions, the release of oxygen was used to determine the enzyme activity by the presence of
The objective of the experiment is to measure and compare the enzyme activity (the enzyme Catalase which is extracted from chicken liver) and the factors that speed up the enzyme action such as concentration of the enzyme, temperature, and pH. This experiment was also used measure the production of oxygen gas using a fermentation vial as it reduces hydrogen peroxide. If the peroxide is destroyed within the experiment, there is a lesser amount available to have a reaction while the oxygen is produced at a lower rate. With that in mind, if there isn't any hydrogen peroxide left; oxygen is no longer created.
The purpose of testing the temperature on the rate of enzyme activity is to examine how different temperature might increase or decrease activity. Adding energy in the form of heat to the enzyme will most likely increase activity because more reactions are able to take place. However, cooling down the enzyme and thus removing energy will most likely slow down the process because there is less energy available for the enzymes to utilize for reactions. However,