Summary: The photosynthesis lab is comprised of three short experiments. These experiments showed how to understand and apply the absorbance spectrum and which colors and wavelengths correspond to visible light. In order to fully understand this absorbance spectrum and how to apply it, we initially prepared a substance comprised of acetone, a large spinach leaf and petroleum ether and measured its absorbance in the spectrophotometer. This showed us what wavelengths corresponded to the most absorption of the spinach leaf. It is understood that the least amount of absorbance should occur after 500 nm and the lower the number, the lower the absorbency. Thus, the 700 ranges is the very least amount of absorbency and the results showed us that the lowest amount of absorbance was around 740nm, which is an accurate, representation of this solution. Next, to understand what wavelengths of light drive the light reactions you can visibly see in photosynthesis, we used CMS in a variety of tubes and measured its absorbency under white light, no light, and colored filters such as red and green. We then used the chlorophyll extract we originally prepared and painted this on a chromatography strip. We measured the band distances to find out the number of pigments in the spinach leaf. These processes helped emphasize how the chloroplast pigment extract and chloroplast membrane suspension have different functional capabilities and how photosynthesis works. Methods: This photosynthesis
The purpose of this lab is to observe the effect of white, green, and dark light on a photosynthetic plant using a volumeter and followed by the calculation of the net oxygen production using different wavelengths color of white and green light, and also the calculation of oxygen consumption under a dark environment, and finally the calculation of the gross oxygen production.
Photosynthesis is a vital process that requires to utilize energy for plants. This experiment was done to evaluate the effects of carbon availability on photosynthetic activity. The aquatic plant Elodea densa was placed into sodium bicarbonate solutions of five concentrations ranging from 0.1% to 1.0%, in five independent trials (excluding the negative control treatment of water). The temperature and light intensity was constant. The results indicated a directly proportional relationship between the availability of carbon dioxide and the rate of photosynthesis of Elodea Densa, as photosynthesis continued to increase with increasing amounts of bicarbonate. The most O2 amount of oxygen produced was with the 0.7% NaHCO3 concentration and least with the control of water. The null hypothesis that stated carbon concentration does not affect rate of photosynthesis of the aquatic plant was rejected. The predicted hypothesis that an increase in bicarbonate concentrations results in an increase in the photosynthetic rate was accepted. In conclusion, there is a significant increase in photosynthetic activity as the concentration of NaHCO3 increases.
Think fast. Think green. What comes to mind when you hear the word photosynthesis? Take a short quiz to see how much you know!
Does carbon concentration affect the rate of photosynthesis? The carbon concentration does affect the rate of photosynthesis.
Photosynthesis is a food making process for algae and plants. The photosynthesis process rate varies from different wavelengths and intensities of light. This lab will evaluate the optimal wavelengths and degrees of intensity during photosynthesis when chloroplast is exposed to light. The mixtures of DCPIP with water, PO4 buffer, and chloroplast will be prepared in a number of cuvettes. The cuvettes were tested individually at different wavelengths and intensities to find the optimal rate of photosynthesis by using a spectrophotometer, measuring the greatest change in absorbance. From this experiment, two data charts and four graphs were obtained. The hypothesis was set from graphs obtained in this lab, and the optimal reaction
The initial experiment was a success. As our treatment group spent more and more time under the lights, the absorbance rate continues to decrease toward zero. Once our 30 minutes were up, the absorbance rate in each tube was significantly lower than at the start of our experiment. In contrast the two control groups did significantly lower the absorbance. Each control lacked one of the vital aspects of photosynthesis, one being light, and the other being chloroplast. Neither of the control groups (Control 1 or 2) showed any signs of photosynthesis. Control 1 was exposed to light, but contained no photosynthetic organelles thus the absorbance throughout the 30 minutes varied minimally, mostly staying stagnant. Control two which contained chloroplast but was not exposed to any light failed to lower the absorbance at all and in fact increased the absorbance over the 30 minutes. However, the treatment group contained both and ultimately performed photosynthesis as we expect therefore, confirming our assumption that chloroplast were the organelles required for photosynthesis in plants and that light is required to perform said photosynthesis. The treatment group, containing both the chloroplast and being exposed to light provided evidence that photosynthesis was taking place as the absorbance lowered at each 10-minute interval. Having a less absorbance would be desired because as DCIP became reduced we would expect the solution to become more and more clear, thus less
Photosynthesis is essential to all living organism such as animals and plants. Photosynthesis is a process used by plants and other autotrophs to capture light energy and use it to power chemical reaction that converts carbon dioxide and water into oxygen, carbohydrates and water. (Textbook: Principles of Biology). The reactants and the products of photosynthesis are:
I am oxygen in the air moving towards a big badger. This badger is going to inhale me and then take me to his lungs, but before this occurs I was a part of another reaction called photosynthesis where I was taken out of the plant and brought into the air. For example, I was a by-product of the first light-dependent reaction of photosynthesis. Photosynthesis is the action of converting sunlight, water, and carbon dioxide into glucose or sugar and myself, oxygen. First, water entered the plant by either absorbing it through the plant’s tissues or coming from the roots and passing through the xylem.
(Photosynthesis. (2008, August 2). Retrieved December 5, 2014, from http://biology.clc.uc.edu/Courses/Bio104/photosyn.htm). The chlorophyll is built into the membranes of the thylakoids. Because chlorophyll absorbs blue and red light waves it appears green. The green light waves that aren’t absorbed give us the ability to see it.
Chlorophyll b absorbs more at 450nm and at 680nm due to its aldehyde side chain. There are carotenoids that soak up light around 400 and 500nm and convert it to reaction centers. Photosystem 2 associates with wavelength shorter than 680nm, it shifts electron to pheophyton and it goes
For lab 12, it is hypothesized that chlorophylls a and b are present in a plant leaf and contribute to the starch production in photosynthesis. Also, products of photosynthesis will be present in leaf tissue exposed to red and blue light wavelengths for several days, but a decreased presence in leaf tissue exposed to green and black light wavelengths. In lab 13, it is expected that since chlorophylls a and b are more polar and smaller molecules than the anthyocyanins and carotenoids, they will travel higher up the chromatography paper than the other pigments.
This lab was called photosynthesis: understanding photosynthesis. It is a highly complex process that needs to be broken down in many steps to understand how it works. This lab covers the big components in photosynthesis including carbon dioxide intake, light consumption, and varying pigmentation.
Without photosynthesis we would not be able to receive energy. We should be more appreciate of plants, without them we would not survive. This paper will explain the basic components require for photosynthesis, the role of chlorophyll, how energy is transferred, and photosystems I and II and the most precious product results of photosynthesis.
Photosynthesis is a biochemical process in which plant, algae, and some bacteria harness the energy of light to produce food. Nearly all living things depend on energy produced from photosynthesis for their nourishment, making it vital to life on Earth. It is also responsible for producing the oxygen that makes up a large portion of the Earth¡¦s atmosphere. Factors that affect photosynthesis are light intensity and wave length, carbon dioxide concentration, and temperature.
Photosynthesis is a very complicated process. It is not as simple as plants need a little sunlight, water, and carbon dioxide, and viola oxygen is produced. There are many steps and processes that occur during photosynthesis which make it very complicated. Now the actual word photosynthesis in Greek means photo- “light”, and –synthesis “putting together”. This is the overall basic foundation that photosynthesis stands behind. Photosynthesis can only happen in plants and some algae, due to them having an organelle called chloroplast. Chloroplast has a pigment, which is called chlorophyll. Chlorophyll is a light absorbing pigment, which allows the plant to control solar energy and use it to distribute energy and food for the plant itself. Chloroplasts are usually located in the green tissue in the interior of the leaf called the mesophyll. A usual cell has around thirty to forty chloroplast. In the inner compartment there is a thick fluid called the stroma, with a system of interconnected membranous