Metabolism refers to the sum of all the necessary chemical processes that allow living organisms to interconvert and use energy to maintain cellular activity. It can be subdivided into two categories, the first process is called catabolism which is the process of breaking down molecules to obtain energy, and the second process is called anabolism which is using energy to synthesize molecules to sustain cellular activity (Mandal et. al, 2013). With the knowledge that all living organisms undergo metabolic processes in order to survive, it is important that we understand the process of metabolism and how specific changes in environmental conditions can affect overall metabolic activity.
The purpose of the present study was to analyze the effect of caffeine on goldfish metabolism by measuring their oxygen consumption rates. We wanted to analyze the effect of caffeine on metabolic processes, because present research shows that administration of caffeine therapy to premature
…show more content…
The second pair of goldfish had a collective mass of 9.35 g (Figure 4), and its oxygen consumption rates in Control Trial 2 and Experimental Trial 2 were 0.0008 and 0.0007 (Figure 5), respectively.
Figure 6 showed the pooled data for all eight groups in the class that used the same control and experimental conditions. The pooled data for the 16 control trials showed that the average oxygen consumption rate per unit mass was -246 and the standard deviation was 87 (Figure 6). The pooled data for the 16 experimental trials showed that the average oxygen consumption rate per unit mass was -263 and the standard deviation was 89 (Figure 6). The t-value derived from the t-test for the average oxygen consumption rate per unit mass was 1.2173, and its corresponding p-value was 0.245 (Figure 6).
According to the results, the columns of caffeine in figure 1, of this experiment the hypothesis for caffeine is partially accepted. There is an increasing trend in the change of pulsation rates with increasing
This lab was conducted with the purpose of confirming the trait of homeostasis among goldfish. During the experiment, it was recorded that the fish would increase gill movement when placed in colder water two out of the three trials. However, the results showed no significant difference in gill movement in various temperatures of water. This has very little effect on the broad field of science since our only three trials were performed and may have included human error in the trials.
At the conclusion of the experiment, the two hypotheses were reviewed. Because the water temperature did affect the normal respiration patterns of the goldfish, the null hypothesis was disregarded and the alternative hypothesis was accepted. From the results of this experiment, it was concluded that although other environmental factors could play
Energy metabolism comes in different types such as Anabolism and Catabolism energy. They all make sure that energy breaks down
All of the chemical processes of the cell are called metabolism. The breakdown or degradation of complex organic molecules to yield simple molecules and energy is called catabolism. Anabolism is the total biosynthetic processes where large complex molecules are made from small simple molecules. Anabolic processes require energy because order is being created and thus work must be done. Overall, both processes of metabolism must occur concurrently because catabolism provides the energy necessary for anabolism.
To determine the metabolic rate of a goldfish two different methods can be applied, direct or indirect calorimetry. Direct calorimetry analyzes the exothermic reaction when ATP is produced by measuring the amount of heat that is released. Meanwhile, indirect calorimetry measures the amount of carbon dioxide or oxygen because both are components of aerobic respiration, a process which repeatedly supplies more ATP to match the demands of metabolic rate of an organism. Evidently, metabolic rate is the cumulative sum of energy used by all the cells. Most of this energy comes from regulating homeostasis, locomotion and thermoregulation. On the other hand, ectotherms like goldfish have a slight difference in their metabolic rates because their internal temperature directly correlates with the temperature of their environment. For this reason, ectotherms use less energy because they do not need to worry about thermoregulation, maintaining constant body temperature. However, temperature, size, amount of light and stimulus are factors that can affect metabolism of goldfish. Thus, this experiment will measure the metabolic rate of goldfish through in
The procedure for this experiment followed the steps as shown in the flow chart (Figure 1) and will be expanded upon here. The oxygen-measuring probe in the test chamber was first turned on and required 10 minutes to warm up. During this time, two empty containers had 200mL of fish water added to them and then each was weighed using a scale that was tared to zero before use. Two pairs of goldfish were then collected from the large tank #1 in the lab room using the fish net provided, and one pair was placed in each container. Then, the containers were reweighed separately and the original weights were subtracted from the new weights in order to determine each pair of goldfish’s weight. Two trials were conducted and in each trial, each goldfish pair was subjected to two conditions, first a control condition where no factors were introduced, followed by an experimental condition where they were exposed
Metabolism is a chemical process that converts fuel from food into energy needed for the body’s activities. For example, thinking, running and jumping etc. are all an example of the bodies activities.
The purpose of this lab report is to discover the exact effect caffeine has on the Daphnia’s heart rate per minute based on the day at my partner and I have collected from the experiment.
In this experiment we find how caffeine can affect the heart rate of a culture Daphnia. Heart rate of a living organism’s can vary depending on the individual, age, body size, heart conditions, medication use and even temperature. This report will examine if the caffeine is good or bad for the living organism’s health and body. And discuss about where the caffeine is produced and used in daily life of human beings and on the environment. Daphnia is a water flea used in this experiment because of its genomic infrastructure with wide range of phenotypic diversity. This quality of Daphnia makes them a versatile model for the experiment. Also their transparent body allows the experimenter to visually see how the heart beats and count them under the light microscope during the experiment as required. The heart rate of Daphnia is monitored under different concentration of caffeine solution and the results are shown in a table and a graph. Experiment carried out to locate the effects of caffeine on a heart rate of Daphnia may or may not be a predictor of change in human heart rate under caffeine. The effects of caffeine can also be tested on humans but those experiment involving humans contains high risk, as Daphnia can only live for a short period of time and in nature most of them get eaten within their first few days or weeks of life.
The null hypothesis for this experiment is that there is no statistically significant relationship between the metabolic rate of goldfish and their exposure to light. The alternative hypothesis is that goldfish exposed to ambient light will exhibit a higher metabolic rate compared to goldfish that are exposed to dark light. Results Overall 86 goldfish (N=43) were used in the experiment. Altogether, 43 trails (2 fish per trial) were conducted in order to track the metabolic rates of the fishes. Differences in metabolic rates (with respect to light exposure) were calculated by measuring the changes of oxygen concentration in the water chambers where the fishes were placed.
For the initial set-up of the experiment, two plastic beakers, each containing approximately 200mL of fish water, were individually weighed and recorded. The selected model organism for the experiment was the Carassius auratus, most commonly known as the goldfish. Each beaker was then filled with two pairs of evenly-sized goldfish and reweighed. These weights were then subtracted by the previously recorded weights to provide an approximation of the combined weights of each paired goldfishes. An oxygen chamber with a built-in probe was then filled with 200mL of fish water. The first pair of goldfish was added, along with the 200mL of fish
In order to determine if the entire class was able to reach the objective, Ms. Meaghan and I took anecdotal notes during our observation groups about student’s observations and quotes about each basic need. In each group, each student was assigned a job, which was described on each card. Their jobs related to providing the goldfish with their basic needs for survival. Three students in each group were feeders, four students were planters, and four were water changers (two added water and two replaced). The feeders each received a piece of scrap paper with some food on it, and each student took turns feeding the fish. The planters each put a sprig of elodea in the tank to create shelter. The two water removers, each took a small Dixie cup of “dirty” water out of the tank, and the two water adders, put one small Dixie cup of conditioned water into the tank. Through their work in their small group, the students were able to develop an understanding of each need of goldfish, and understand how to provide it. They were able to have a chance to actually provide the
The purpose of this lab report is to determine the effects of a different environment condition such as temperature on the consumption of oxygen and ventilation in goldfishes (Carassius auratus). The consumption of oxygen and ventilation rate was measured in goldfishes at different treatment levels inside the tanks; ~ 25°C and ~15°C in a 15 minute time interval for one hour. At ~ 25°C, the ventilation rates in the goldfishes were higher than at ~ 15°C and there was more oxygen consumption at ~ 15°C than at ~ 25°C. The results suggest that the ventilation rate is greater in high temperature than low temperature. Also, there is more oxygen consumption at lower temperature than at higher temperatures. This experiment shows that temperature is
We could go even depeer in the explanation of metabolism reaction but this is just an