Lab 5
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LAB #5: Bradford Assay Worksheet, Fall 2022 1. Complete the tables below: BSA STANDARD CURVES Calculate how much BSA is actually being read in the spectrophotometer and write it down in the worksheet below. In other words, you prepared a 1000 µL solu,on of the standard protein, but you only pipeLed 5 µL into each well to read. How much protein is actually in the well? (2 pts) [BSA] (mg/mL)
Volume IgG stock (ml)
Well #
A595
Well #
A595
Average A595
0.0
A1
B1
A1, B1
0.1
A2
B2
A2, B2
0.2
A3
B3
A3, B3
0.3
A4
B4
A4, B4
0.4
A5
B5
A5, B5
0.5
A6
B6
A6, B6
0.6
A7
B7
A7, B7
0.7
A8
B8
A8, B8
0.8
A9
B9
A9, B9
0.9
A10
B10
A10, B10
1.0
A11
B11
A11, B11
1
Name:___________________________________ Date:_________________ Sec,on#:_____________
IgG STANDARD CURVE (2 pts) 2) Using the posted “How to Create the Standard Curve” guide, plot standard curves for BSA or IgG. Alterna,vely, you can design your own spreadsheet or other computer program to generate a standard curve or you may draw one by hand. (3 pts) [IgG] (mg/
mL)
Volume BSA stock (ml)
Well #
A595
Well #
A595
Average A595
0.0
D1
E1
D1, E1
0.1
D2
E2
D2, E2
0.2
D3
E3
D3, E3
0.3
D4
E4
D4, E4
0.4
D5
E5
D5, E5
0.5
D6
E6
D6, E6
0.6
D7
E7
D7, E7
0.7
D8
E8
D8, E8
0.8
D9
E9
D9, E9
0.9
D10
E10
D10, E10
1.0
D11
E11
D11, E11
2
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Check
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Click and drag to start drawing a
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$
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%
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6
::
&
7
X
S
* 00
to
v
© 2023 McGraw Hill LLC. All Rights Reserved. Terr
(
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1. After reading chapter 9 from your textbook, you will be able to explain scatter radiation and how it is produced as a result of the
attenuation of the x-ray beam by matter. You will also recognize the factors that influence its formation and how to minimize the fog that
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NCCT
Note: Please read the instructions provided in the following links before submitting your entry:
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3. Recombinant protein is produced by a genetically engineered strain of Escherichia coli during cell
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secreted from the cells; it is synthesized in addition to normal E. coli biomass. Ammonia is used as
the nitrogen source for aerobic respiration of glucose.
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recombinant protein) from glucose is measured as o.48 g/g; the yield of recombinant protein from
glucose is about 20% of that for cells.
How much ammonia is required?
What is the oxygen demand?
If the biomass yield remains at 0.48 g /g, how much different are the ammonia and oxygen
requirements for wild-type E. coli that is unable to synthesize recombinant protein?
а.
b.
с.
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08:39
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27/04/2022
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Chapter 7
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Recommended textbooks for you
- Case Studies In Health Information ManagementBiologyISBN:9781337676908Author:SCHNERINGPublisher:Cengage
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