You are a new graduate student working in a research lab with a strain of E. coli. Before you get started with your project, your research advisor wants to know if you understand carbon metabolism in the E. coli strain you are working with. You can assume that E. coli has all of the components it needs to perform glycolysis, the conversion of pyruvate to acetyl-CoA, the TCA cycle, the electron transport chain, and fermentation. What is the highest net total ATP (accounting for both energy input and output) that your E. coli strain can produce when grown aerobically on Phosphoenolpyruvate Minimal Media? Provide the total based on the input of 1 molecule phosphoenolpyruvate. The input cost for Phosphoenolpyruvate is 1 ATP

You are a new graduate student working in a research lab with a strain of E. coli. Before you get started with your project, your research advisor wants to know if you understand carbon metabolism in the E. coli strain you are working with. You can assume that E. coli has all of the components it needs to perform glycolysis, the conversion of pyruvate to acetyl-CoA, the TCA cycle, the electron transport chain, and fermentation. What is the highest net total ATP (accounting for both energy input and output) that your E. coli strain can produce when grown aerobically on Phosphoenolpyruvate Minimal Media? Provide the total based on the input of 1 molecule phosphoenolpyruvate. The input cost for Phosphoenolpyruvate is 1 ATP

Concepts of Biology

1st Edition

ISBN:9781938168116

Author:Samantha Fowler, Rebecca Roush, James Wise

Publisher:Samantha Fowler, Rebecca Roush, James Wise

Chapter4: How Cells Obtain Energy

Section: Chapter Questions

Problem 17CTQ: Both prokaryotic and eukaryotic organisms carry out some form of glycolysis. How does ha fact...

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Draw the pathway for the conversion of Propionyl CoA to Succinyl CoA as it occurs in thematrix of the mitochondria. Show the structures and names of all reactants and products, as well asthe names of the enzymes. You do not need to draw the structures of ATP, NAD+, CoA, etc., but doshow them as reactants or products in the appropriate places. You do not need to show themechanisms of the enzymes. Draw the pathway for the conversion of Succinyl CoA into 4 CO2. Show the structures andnames of all reactants and products, as well as the names of the enzymes. You do not need to drawthe structures of ATP, NAD+, CoA, etc., but do show them as reactants or products in the appropriateplaces. You do not need to show the mechanisms of the enzymes. How many ATPs would be generated by the complete oxidation of Propionyl CoA into 3 CO2using any of the required enzymes of -oxidation, gluconeogenesis, glycolysis,the citric acid cycle andoxidative phosphorylation?
I've stated that acetyl-CoA is perhaps the most central compound to our metabolic processes. Explain why I would make such a bold statement in the context of the metabolic processes we've covered so far.
Cyanide poisoning inhibits aerobic respiration at cytochrome c oxidase. Which of the following is NOT a result of cyanide poisoning at the cellular level? Select an answer and submit. For keyboard navigation, use the up/down arrow keys to select an answer. a b с d e Oxygen is reduced to water The rate of glycolysis increases Cells are forced to switch to anaerobic respiration The electron transport chain is not completed None of the above Answered K Open in Reading View ✔Posubmit
Consider the steps of the krebs cycle and electron carrier chain and answer: a) How many ATPs are produced from 10 Pyruvato molecules? b) How many ATPs are produced from 12 Acetyl CoA molecules?
Lactic acid is a biproduct formed at the end of anaerobic respiration. This molecule is dangerous to cells of the body if it builds up too much.Despite this, the formation of lactic acid is very important. Explain why the process of anaerobic respiration is so important (providing reference to glycolysis, the role of coenzymes, and ATP formation). The answer should show in detail the stages of all the steps of anaerobic respiration with detail of location, key products etc.
Both prokaryotic and eukaryotic organisms carry out some form of glycolysis. How does ha fact support or not support the assertion that glycolysis is one of the oldest metabolic pathways?
Look at this molecule and determine the number of molecules created from B-Oxidation: FADH2, NADH + H, Acetyl-CoA, Propionyl-CoA, Formyl-CoA. What is its net ATP and how did you calculate it?
Both prokaryotic and eukaryotic organisms carry out some form of glycolysis. How does that fact support or not support the assertion that glycolysis is one of the oldest metabolic pathways?
A fatty acid (a long straight-chain carboxylic acid with an even number of carbons) is metabolized to acetylCoA, which can then enter the citric acid cycle to be further metabolized. A fatty acid with an odd number of carbons is metabolized to acetyl-CoA and one equivalent of propionyl-CoA. Propionyl-CoA cannot enter the citric acid cycle. Two coenzyme-requiring enzymes are needed to convert it to succinyl-CoA, a compound that can enter the citric acid cycle. Write the two enzyme-catalyzed reactions and include the names of the required coenzymes.
Identify different types of organic reaction mechanims in the followingmetabolic pathways.1. Conversion of Pyruvate to Acetyl CoA2.Citric acid cycle3. Gluconeogensis pathway (pyruvate to glucose)
Create a metabolic pathway map that includes "the 4 Gs": glycolysis, gluconeogenesis, glycogen synthesis & glycogenolysis, as well as one type of fermentation, and pyruvate oxidation (to acetyl-CoA). The map should show all these 6 pathways on the same page, to emphasize how these processes are related to each other. Your map should include: a) Clear labels for all the pathways b) All the pathways shown on the same page and correctly integrated with each other, i.e., it should be clear which reactions are shared by different pathways c) The names of all metabolites (common abbreviations may be used) d) The names of all enzymes e) All relevant cofactors/co-substrates discussed in class, clearly showing, where ATP is used and produced f) Double or single arrows representing reversible or irreversible reactions, respectively g) All "high-energy" intermediates clearly labeled with an asterisk (*) h) Labels for cellular locations of the pathways/reactions
Medical researchers are studying a patient with the newly discovered u LEKS metabolic syndrome, in which a genetic mutaton prevents NADH produced in the cytosol from being reoxidized by the electron transport chain. Suppose the following nutrient molecule is digested and metabolized by this patient under anaerobic conditions, e.g. during a sudden intense exertion: de HO-CH: -OH Он н. HO H OH How many ATP molecules will uttimately be produced by the catabolism of this molecule? Note: you can ussume the catabohism is as complete as it can be under the conditions given above. How many molecules of pyruvate will be produced during dycalysist How many maleoules of lactate will be produced