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- If you performed the serial dilution using 0.9ml of tapwater originally and mixed and transferred 0.1 ml each time, what would be your dilution in tube 8?Draw a serial dilution that would give a dilution of 1/153 000 000. Each tube in your dilution series must have a volume of 50 ml or less.Calculate the final dilution in the following dilution series: a. 1 mL in 9 mL dilution & 100uL spread onto the plate b. 1 mL in 9 mL dilution followed by a 100ul in 900ul dilution & 100uL spread onto the plate (note bacteria are counted on plates as cfu/mL)
- To the right is an image of a dilution that was performed. The volume above the top arrow indicates the volume that should be transferred to the next tube (i.e. Tube 1). The volume listed at the bottom-right of tube 1 indicates the volume of diluent that should be added to that tube. The stock concentration is 50μg/ml and you want to make a solution in tube 1 with a concentration of 0.4μg/ml and a total volume of 3ml. Stock ?ml Tube 1 ?ml a. How many milliliters of stock solution needs to be added to Tube 1? Round your answer to three decimal places (e.g. 0.111). b. How many milliliters of diluent needs to be added to Tube 1? Round your answer to three decimal places.Propose a serial dilution that would give a dilution of 1/153 000 000. Each tube in your dilution series must have a volume of 50 ml or less.Complete the dilution series using a maximum amount of 20 mL of dilutent so that the dilution in the final tube is 10^-9
- Propose a serial dilution that would give total dilution of 1/158000000 (six zeros) Each tube in your dilution series must have a volume of 50 ml or less.If you transferred 15 mL of an undiluted sample into 35 mL of diluting fluid, what dilution have you created? (Note: provide your answer in decimal format to three decimal places.)fill in the blank (3 blanks for 3 tubes) with the correct dilution for each tube in the series.
- 6) 1 mL of supernatant is required for a procedure. The final colored solution proves to be too high to read accurately on the spectrophotometer. 100 μL of supernatant and 900 μL of distilled water are substituted for the original supernatant and the procedure, run as before. The reading from the standard curve is 46 mg/dL. What is the actual amount of substance in the patient serum?How do you calculate the initial concentration of a sample? How would you make a 1:10 dilution of a broth culture if the final volume of the dilution is 10 ml? How much broth, and how much diluent, would you add? If you made a series of tenfold dilutions, exactly the same way as you described in question 1, what would be the dilution factor in the fifth tube? The following are errors that people commonly make when they perform serial dilutions. Indicate whether you think that the number of cfu/ ml calculated would be too high or too low if you make this mistake. You intend to add 0.9 ml of diluent to each tube and 0.1 ml of culture. Instead, you add 0.5 ml of diluent to each tube and 0.1 ml of culture to the first tube. Then, you make a serial dilution of 0.1 ml into and from each tube as described. You prepare 0.9 ml of diluents in each tube. You add 0.1 ml of culture (from the overnight culture provided) to every tube. You add 0.9 ml of diluent to each tube. You add 0.1 ml of…Label the void volume (V0), elution volume (Ve) and total elution volume (Vt) appropriately on the graph for the gel filtration chromatography using a column experiment.