Please indicate true or false about the statements regarding flies with the following genotype: Hs>FLP; FRT82B, Actin-GFP, e2f1i2. e2f1i2 is a mutation and that both this mutation and Actin-GFP reporter are located on the same chromosomal arm as FRT82B. This line can be crossed with w; FRT82B to create mosaic clones in wing discs after heat shock. Cells with higher GFP intensity correspond to the WT and the lower intensity GFP-positive cells are heterozygous. When this line was crossed with w; FRT82B males, no mosaic clones will be generated in wing discs even after heat shock. All the cells in wing discs will have similar level of GFP expression. This line can be crossed with w; FRT82B to create mosaic clones in wing discs after heat shock. Cells with no GFP expression can be used to identify the WT cells. This line can be crossed with w; FRT82B to create mosaic clones in wing discs after heat shock. Cells with no GFP expression can be used to identify the e2f1i2 mutant cells.

To answer these statements, let's break down the genotype: Hs>FLP; FRT82B, Actin-GFP,…

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Human Heredity: Principles and Issues (MindTap Course List)

11th Edition

ISBN:9781305251052

Author:Michael Cummings

Publisher:Michael Cummings

Chapter11: Genome Alterations: Mutation And Epigenetics

Section: Chapter Questions

Problem 20QP: The cystic fibrosis gene encodes a chloride channel protein necessary for normal cellular functions....

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Please indicate true or false about the statements regarding flies with the following genotype: Hs>FLP; FRT82B, Actin-GFP, e2f1ⁱ². e2f1ⁱ² is a mutation and that both this mutation and Actin-GFP reporter are located on the same chromosomal arm as FRT82B.

This line can be crossed with w; FRT82B to create mosaic clones in wing discs after heat shock. Cells with higher GFP intensity correspond to the WT and the lower intensity GFP-positive cells are heterozygous.

When this line was crossed with w; FRT82B males, no mosaic clones will be generated in wing discs even after heat shock. All the cells in wing discs will have similar level of GFP expression.

This line can be crossed with w; FRT82B to create mosaic clones in wing discs after heat shock. Cells with no GFP expression can be used to identify the WT cells.

This line can be crossed with w; FRT82B to create mosaic clones in wing discs after heat shock. Cells with no GFP expression can be used to identify the e2f1ⁱ²mutant cells.

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