You've crossed two strains: (Hfr) leu+ his+ bio+ with (F-) leu-his-bio- . Interrupted mating studies indicated that leu+ enters the cell last, so you want to select for leu+ recombinants in order to map the genes by recombination. The following numbers are found for each genotype: leu+ his+ bio+ : 650 leu+ his+ bio- : 0 leu+ his bio+ : 14 leu+ his bio- : 84 Determine the gene order. Which gene is in the middle?

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Mitochondrial Mutations

A mitochondrion is a cellular organelle that serves as the site for cellular respiration and energy production (ATP or Adenosine Triphosphate). It is a double-membrane-bound organelle and is only present in eukaryotic cells.