You wish to quantify your favourite compound in an experimental solution using spectrophotometry. First, you use standard solutions of your favourite compound to collect the following standard curve data. Calculate the slope of the line of best fit to two decimal places, and enter it in the box below the table.
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- What are the x and y values that you have input in the excel? I thought the x would be from the concentation and the y is from the A595 of sample minus A595 of blank but the linear equation that shows up in mine is different. It's y = 0.0443x + 0.0148.Why is it important that the standard curve you create in biological analyses with spectrophotometry is measured using specialty cuvettes?I need assistance with how to read Fletcher Munson graphs. Please help and show me the proper way to analyze these graphs. The previous experts I've asked on this platform were incorrect.
- * You can keep extra decimal places through most of this problem, but round your final estimate to one decimal place* You wish to quantify your favourite compound in an experimental solution using spectrophotometry. First, you use standard solutions of your favourite compound to collect the following standard curve data. Calculate the slope of the line of best fit. Standard curve data Conc (mM) 0.300 0.600 0.900 1.200 1.500 Abs 0.150 0.300 0.450 0.600 0.750 Next, you prepare an experimental sample by mixing 0.50 mL of the compound solution with 9.50 mL of distilled water, and you read an absorbance of 0.625 for this sample. Calculate the concentration (mM) of the original solution of compound. Round this answer to one decimal place.The actual length of a cell is 50µm. When projected on the big screen in the class, the same cell is 2m long. What is the magnification factor of the cell on the screen? Practice O 1 000x O 4 000x O 10 000x O 40 000x 100 000x O400 000xBriefly explain how the API (Analytical Profile Index) system works.
- Calculate the total surface area (m^2) of the entire sampling plot (all the tree quadrats) (no units)Here are the materials and method for the basic spectrophotometer experiment Materials:1. Paper template2. Scissor3. Light source (i.e. torchlight, portable light)4. Paperboard (black colour)5. Unused compact disc (CD)6. Camera detector (i.e. webcam, laptop, mobile phone) Methods: 1. Make a foldable paper spectrophotometer using the paper template and followthe instruction as in https://publiclab.org/sites/default/files/8.5x11minispec3.8.pdf2. Then, mounted the paper spectrophotometer to the camera detector.3. Download any freely available spectral analyzer.4. Test and observe the wavelength with and without the presence of a light source.5. Finally, observe the wavelength with a different transparent colour sheets (you canchoose any colour). Troubleshooting/ problem(s) encountered for basic spectrophotometer experiment.Calculate the amount of phycocyanin in Sample 1 in mg where A620=0.204 and A650=0.061, taking into account the dilution factor as per question 6, and the total volume of extract as per question 4. Note your answer to 2 decimal places Sample 1 Total Extract Volume (ml) is 45 Dilution factor is 100 A280 is 1.07 A620 is 0.221 A650 is 00.97 Specific Absorbance Ratio (SAR) A620/A280 is 0.206
- Metric Prefixes Nano-, Micro-, Centi-, and Milli- Match each prefix with its value. 1 Micro 2 Nano Centi 4 Milli Match each of the options above to the items below. 1 x 10-3 No answer 1 1 x 10-2 2 3 4 1x 10-9 1х 10-6 mIn regards to an experiment where paper chromatography is used to calculate the Rf number, is this considered a qualitative or quantitaive assessment of the data? Why is it a qualitative or quantitative assessment of the data?Suppose a researcher is investigating the measurement ability of a new device intended to read the freezing point of a chemical compound. The substance used in the investigation has a known freezing point of -24 degrees Celsius. The researcher conducted a series of 10 sample measurements of the freezing point, and the results are represented below. What can we say about the device with regards to its precision, and potential bias? Measurement Freezing Point (degrees Celsius) 1 -26 2 -18 3 -32 4 -31 5 -24 6 -24 7 -24 8 -24 9 -11 10 -37