You have performed an experiment and your aim is to obtain pure colonies. However, you observe some colony variations and you need to verify that this is not due to contamination, name and describe the staining method would you use to achieve this goal?
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- You have identified two colony types A and B on the streak plate. Now briefly describe (in 3-4 sentences) the process of how you identified the cellular morphology/arrangement of each isolate. Again, assume you have all the necessary equipment and materials at your disposal. Be concise and thorough, not verbose; e.g., refer to the Gram stain, but describing the details of each step is not necessary.Briefly, in your own words, describe the steps of the gram staining procedure.Select all that apply to a direct simple stain: 1. the dye is acidic or negatively charged and stains the background 2. uses multiple dyes in the procedure 3. cells will never be distorted or shrunken 4. uses only 1 dye in the procedure 5. does involve a washing step 6. can be used to determine cell morphology, size, and arrangement. 7. does involve fixing 8. the dye is basic or positively charged and stains the bacteria 9. cells may be distorted or shrunken 10. does not involve a washing step 11. does not involve fixing
- You have identified two colony types A and B on the streak plate. Now briefly describe (in 3-4 sentences) the process of how you identified the cellular morphology/arrangement of each isolate. Again, assume you have all the necessary equipment and materials at your disposal. Be concise and thorough, not verbose; e.g., refer to the Gram stain, but describing the details of each step is not necessary. Following the description, provide the cell morphology information for each isolate. Isolate A – Describe: Cellular morphology & arrangement: Gram stain color & result: Isolate B – Describe: Cellular morphology & arrangement: Gram stain color & result:Select all that apply to a negative stain: 1. involves a washing step 2. cells may be distorted or shrunken 3. uses an acidic or negatively charged dye which stains the background 4. uses multiple dyes in the procedure 5. uses only 1 dye in the procedure 6. involves fixing 7. does not involve fixing 8. cells will not be distorted or shrunken 9. does not involve a washing step 10. can show cell morphology, size, and arrangement 11. uses a basic or positively charged dye which stains the bacterial cellsWhat staining method would you use to verify that the isolate is not producing survivalstructures against unfavourable conditions? Describe the procedure as well
- When analyzing your results after performing a serial dilution, you obtain an average of 40 colonies on your 1 x 106 plate. How many cells were in the original sample?Describe the process to make a 4-phase streak plate beginning with your first streak (i.e you have bacterial culture on your sterile loop and are about to start to streak your agar plate ).Take a look at your streak plate from the use of a mix culture. Does your plate show the results that are expected? If yes, what does this result tell you if no, what error do you think could’ve caused this? Explain.
- This part of the staining process helps the primary stain to remain in the cell during decolorization. O 1) Secondary stain O 2) primary stain O 3) counterstain O 4) fixing reagent O 5) mordantCan you show the step by step process of Methyl Red test using a schematic diagram?Why is it important to use a sterilized loop between streaks when preparing a streakplate? Observation of a streakplate culture shows more growth in Quadrant 4 than in Quadrant 3. Account for this observation. Describe the way in which you can isolate an individual colony from a spreadplate or a streakplate that holds multiple colonies. Outline the differences between a streak plate and a spread plate.