You have counted a total of 6.4 x 10° cells /ml in preparation for plating cells at a concentration of 1.5 x 10° cells/ml in a T cell proliferation assay. You need 5 ml of cells for your assay. Calculate the volume of counted cells and the volume of cell culture medium you will need to mix together to prepare the cells for plating. O 2.73 mi of cells and 2.27 ml medium O 21 ml of cells and 2.9 ml medium O 1.17 ml of cells and 3.83 ml medium O 145 ml of cells 3.55 ml medium
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- 1.a)What is the equivalence point and how does it relate to the recommended proportion of serum to blood in the heme agglutination assay? b. what would the predicted outcome be if you used too little serum in this assay? Why? c. what would the predicted outcome be if you used too much serum in assay? Why?What are the different categories of cell viability assays? Describe one of them briefly. Define the role (aim of use) of Trypan blue dye in cell culture studies.Discuss and explain the results of this graph. Graph shows results of WST-1 assay. T0 ( time zero) shows assay performed in order to obtain an absorbance at the time of test agent added in order to determine 1. how much cells have grown over the incubation period 2. Growth inhibition by test agent 3. If test agent caused cytotoxicity
- During incubation, prepare dilutions of the standard antiserum which constitutes the standard curve for the assay (concentration in ng/mL). Add 500 µL of PBS-milk to the supplied microtube containing 500 µL of standard antiserum to obtain Standard 1 [500 ng/mL]. Identify seven microtubes for standards 2 to 7 and place 500 µL of PBS-milk in each. Calculate how much of antiserum is used in each standard ?Calculate the rough cost estimation of manufacturing 250g of antibodies in 50 L bioreactor using CHO cells. 100,000 cells can produce 100 ug of antibody. Please include: How many cells that you need? Will you need to use microcarrier? How much media is required? Cost of materials: Labor: Time:How does a rapid test detecting RSV work and what kind of binding does it use (competitve, sandwich, indirect)? Explain using anti-rsv antibody conjugated with gold nanoparticle, anti-rsv antibody, and anti-human IgG. Draw a diagram if possible.
- A phagehunter performs a spot titer using standard techniques (3 ul of each dilution spotted to lawn) of a lysate obtained from an optimum webbed plate experiment. The phagehunter counts 4 plaques on the 10-8 dilution spot. Which of the following scenarios is the best choice for the phagehunter to do next? a.) The phagehunter has not achieved a high enough titer lysate to move forward with characterization experiments, so they should try to make more optimum webbed plates. b.) The phagehunter has not achieved a high enough titer lysate to move forward with characterization experiments, so they should adopt a phage from direct isolation. c.) The phagehunter has achieved a high enough titer lysate to move forward with characterization experiments. d.) The phagehunter has not achieved a high enough titer lysate to move forward with characterization experiments, so they should go back to the pick-a-plaque experiment.You want to subculture your cells from T25 flask to 96-well plates. You first collected your cells in a tube with 5ml of culture medium. Then carried out a trypan blue assay and counted your cells with a hematocytometer as shown in Figure below. Answer the questions according to the results: a. Calculate the concentration of the stock including the dead and living cells. Dont forget to show the units! b. Calculate the percentage (%) of the living cells in the stock. c. You want to seed 6000 living cells into each well of 96 well plates, then calculate the volume you should take from the stock for each well. 輯 1 mm I IREA phagehunter performs a spot titer using standard techniques (3 ul of each dilution spotted to lawn) of a lysate obtained from an optimum webbed plate experiment. The phagehunter counts 8 plaques on the 10-7 dilution spot. Which of the following scenarios is the best choice for the phagehunter to do next? a.) The phagehunter has not achieved a high enough titer lysate to move forward with characterization experiments, so they should go back to the pick-a-plaque experiment. b.) The phagehunter has not achieved a high enough titer lysate to move forward with characterization experiments, so they should adopt a phage from direct isolation. c.) The phagehunter has not achieved a high enough titer lysate to move forward with characterization experiments, so they should try to make more optimum webbed plates. d.) The phagehunter has achieved a high enough titer lysate to move forward with characterization experiments.
- A medical technology intern was assigned to the serology section of a clinical laboratory. His supervisor asked him to prepare a 5% red cell suspension, which will be used to run a hemagglutination inhibition assay. The intern, being aware of the importance of preparing the desired concentration of red cells recommended in the assay, considers the factors enumerated on the table below during the sample processing. If you are the intern assigned to the task, explain why these factors are vital to the procedure and determine their effect on the sensitivity of the test. Factors to Consider Importance Effect 1. Tonicity of the suspending saline solution 2. Overfilling of the tube 3. Proper washing of red cells 4. Too heavy or too diluted red cell suspensionFollowing is the data and notice that it is a terrible idea to culture hMSCs longer than 10 days. You’re strongly Days # cells0 50001 75002 125003 125004 218005 287006 530007 1143008 1653009 19200010 19200011 11680012 8950013 8830014 78300 Part1 You are working for a start-up that is pursuing a clinical trial. The trial involves grafting hMSCs intopatients suffering from interveterbral disc disease using a degradable polymer scaffold. You are going to 3Dprint a porous cylindrical scaffold that is 2 cm in radius and 1 cm in height (matching the dimensions of adegenerated disc). Assume a porosity of 50%. You will fill available volume of the scaffold with hMSCs at adensity of 1 million cells per cm3. Based on the data above, what starting number of cells will you use andhow long will it take you to get enough cells for the trial? Part2The trial is a failure (patients did not report any reduction in back pain). Your team wants to try againusing 85% hMSCs and 15% nucleus pulposus cells .…Briefly explain why this is ELISA referred to as colorometric assay.