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You are purifying an enzyme and notice that your sample has a lower activity at each step, yet the specific activity increases. How is this possible?

You are purifying an enzyme and notice that your sample has a lower activity at each step, yet the specific activity increases. How is this possible?

Introductory Chemistry: An Active Learning Approach
Introductory Chemistry: An Active Learning Approach
6th Edition
ISBN:9781305079250
Author:Mark S. Cracolice, Ed Peters
Publisher:Mark S. Cracolice, Ed Peters
Chapter22: Biochemistry
Section: Chapter Questions
Problem 22.3TC
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You are purifying an enzyme and notice that your sample has a lower activity at each step, yet the specific activity increases. How is this possible? Select one: This is not possible, one of the measurements must be wrong ob. The specific activity was lower earlier because excess enzymes tend to oligomerize and fall out of solution O C Specific activity increases when enzymes have fewer of the same enzyme competing for activity O d. None of the above A contaminant protein is being removed by the purification After determining the molecular weight of a protein sample, you want to elucidate the exact sequence of this protein. You run a tandem MS and find one of the amino acids near the middle of the amino acid sequence could either be leucine or isoleucine. How would you go about finding whether the residue is leucine or isoleucine in the most efficient manner? Select one: Oa Continue performing Edman's degradation until you reach that residue b. Conjugate a dye specifically for leucine or isoleucine O C. Run an SDS-PAGE d. Perform a pepsin cleavage e. Perform a trypsin cleavage
Transcribed Image Text:You are purifying an enzyme and notice that your sample has a lower activity at each step, yet the specific activity increases. How is this possible? Select one: This is not possible, one of the measurements must be wrong ob. The specific activity was lower earlier because excess enzymes tend to oligomerize and fall out of solution O C Specific activity increases when enzymes have fewer of the same enzyme competing for activity O d. None of the above A contaminant protein is being removed by the purification After determining the molecular weight of a protein sample, you want to elucidate the exact sequence of this protein. You run a tandem MS and find one of the amino acids near the middle of the amino acid sequence could either be leucine or isoleucine. How would you go about finding whether the residue is leucine or isoleucine in the most efficient manner? Select one: Oa Continue performing Edman's degradation until you reach that residue b. Conjugate a dye specifically for leucine or isoleucine O C. Run an SDS-PAGE d. Perform a pepsin cleavage e. Perform a trypsin cleavage
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