Q: What is the purpose of fixing a smear?
A: Smear- a smear is a thin layer of a sample such as bacterial cells or blood spread evenly on a glass…
Q: Do you think UVC disinfection of N95 face masks is effective? Give two important considerations for…
A: At the time of Covid-19, N95 masks are currently considered critical supplies by the centers for…
Q: Why is a block of agar inoculated rather than streaking the fungal culture on a plate?
A: The culture medium or growth medium is a liquid, solid, or semisolid designed to support the growth…
Q: Does the Gram stain provide information regarding a bacterium's susceptibility to antimicrobial…
A: The Gram stain is performed for the determination and classification of the bacteria. In Gram…
Q: Why bleach disinfectant will the most effective against probiotic?
A: Probiotics are live microorganisms that have health benefits when consumed. These are usually…
Q: What is the purpose of Thick smear? Purpose of Thin smear?
A: Blood smear:-Also known as peripheral blood smear. -Blood smear has many different uses ranging from…
Q: in the clinic, the acid-fast stain is used to diagnose infection with which bacterium?
A: The Ziehl-Neelson (ZN) stain is the most commonly utilized of the different types of AF staining…
Q: Determine the microbiology tests used to identify the microorganism(cutaneous anthrax) i. Gram…
A: Cutaneous anthrax is the most common form of anthrax infection, and it is also considered to be the…
Q: Why was a blood agar, rather than a nutrient agar, plate used for the culture from your mouth?
A: Cultured bacteria are any bacteria that grow in the lab by the microbiologist using the required or…
Q: What do you mean by ultrafiltration?
A: The excretory system is the system that performs excretion, the bodily process of discharging…
Q: Which microbe requires serum components to be added to the growth medium?
A: Some microbes are nutritionally fastidious and hence needs some substances to be added to the growth…
Q: describe the agar-overlay technique
A: Biological techniques are processes or methods for trying to study living things. They include…
Q: How do we prepare smear
A: The preparation of a smear is required for many laboratory procedures, including the Gram-stain, for…
Q: Is UVC disinfection of N95 face masks effective? Give two (2) important considerations for UVC…
A: Decontaminating masks could be effective against the shortage of masks in pandemics.UVC…
Q: Where should a label be written on an agar plate?
A: The aseptic technique is applied in a laboratory setup to reduce the risk of contamination. It…
Q: What are the equipments/ materials used in imoobilizing and separating cells when using: spread…
A: Aseptic techniques refer to any method which is used to sterilize and maintain sterility of the work…
Q: What is the difference between streak plate method and spread plate technique?
A: Answer of the question given below...
Q: I Why does't a negative stain colorize the cells in the smear?
A: Staining is a technique which is used for enhancement of contrast in samples mostly at the level of…
Q: Is it important to have a standardized way of counting and reporting AFB seen on a direct sputum…
A: Acid-Fast Bacilli (AFB) is a bacterial species that causes tuberculosis in humans and testing of…
Q: Why should culture media after inoculation be incubated at an optimal temperature immediately?
A: Culture media, also called as growth media, are particular combinations of nutrients and other…
Q: Explain why the media where the Staphylococcus aureus is plated has turned yellow but the media…
A: Mannitol Salt Agar (MSA) is a differential and selective medium. A member of the staphylococcus…
Q: Can you please help me describe the colony apperance on these two stains? Thank you! Growth on Blood…
A: The chocolate agar and the blood agar media both have the almost same composition. The only…
Q: Why is it important to use sterile practices when working with microorganisms?
A: Microscopic organisms such as bacteria, fungi (mold and yeast), protists, archaea, algae,…
Q: Bacterial motility soft agar method does the test tell a microbiologist the number or distribution…
A: Bacterial Motility: it is defined as the ability of a bacteria to move alongside with the help of…
Q: Why might viable cell cultures be of more use in microbialtaxonomy than preserved specimens?
A: Microbial taxonomy is a field of biological sciences that deals with the grouping of microorganisms…
Q: Why are traditional tests such as metabolic test not always used to ID clinically important…
A: Traditional methods for identification of microbes include phenotypic tests or biochemical tests…
Q: Explain why it is important to use only a small amount of bacteria when preparing a smear
A:
Q: What are the principle and basic concepts of SMEAR PREPARATION? (please explain it thoroughly in a…
A: Introduction A smear is a small amount of culture spread in a very thin film on the surface of the…
Q: If you prepare culture media plates 2 days ahead of the laboratory schedule, can you still use the…
A: Preparation of culture media and inoculation are two of the very sensitive steps in any…
Q: purpose of using serum in culture media
A: A culture media is a media made in the lab, that is curated to facilitate the culture and growth of…
Q: What is selective medium?
A: Description of a selective medium.
Q: Why are smears of capsulated bacteria not heat-fixed before staining as opposed to Gram staining?
A: Gram staining is a differential staining technique developed by Danish bateriologist Hans Christian…
Q: is staphylococcus aureus cells under 40 times magnification bigger than onion cell or cheek cell?
A: Staphylococcus aureus is a Gram-positive, round-shaped bacterium that belongs to the Firmicutes and…
Q: Is UV treament more or less effective than disinfection when used against Saphylococcus aureus?
A: Saphylococcus aureus is a gram-positive bacterium that is found in the human nose, skin, and…
Q: Do you think UVC disinfection of N95 face masks is effective? Give two (2) important considerations…
A: N95 filtering facepiece respirator or N95 mask is a piece of personal protective equipment that…
Q: Why is 15 minutes generally used for sterilization? Why is agar added to medium?
A: Sterilization is the process in which all life forms like microbes (fungi, bacteria, viruses,…
Q: How can moist heat sterilization can sterilize antibiotic solution?
A: Moist heat sterilization is a technique that uses heated, increased pressure steam to sterilize an…
Q: if there are features of the gram stain that are not as expected what possible sources or error may…
A: Gram staining is a general technique that is used to differentiate two larger groups of bacteria…
Q: microbial sensivity lab: in the procedure used to test bacterial growth against various temperatures…
A: Growth of bacteria differs with varying temperature. Bacteria grows rapidly in the temperature range…
Q: How does the agar dilution method differ from streaking toobtain isolated colonies?
A: Both these are methods to culture microorganisms and have their respective pro and cons as mentioned…
Q: What things can be done during sample collection of a sick patient to help eliminate the culture of…
A: Normal flora is the organisms that are present in the body for normal functioning. The normal flora…
Q: What microbe requires serum components to be added to the growth medium?
A: Introduction Microorganisms:- An organism that can be seen only through a microscope, They live in…
Q: If zn stain is positive from a sputum sample, what would be your pre-treatment of choice to isolate…
A: Zn stain test also called as Ziehl-Neelsen test is a rapid method to detect the acid fast bacteria.
Q: Why is low microbial population in milk samples a limitation for microscopic count?
A: The bacteria present in milk are lactococcus , lactobacillus, streptococcus, leuconostoc and…
Q: Why should freshly collected samples be used for microscopic examination?
A: The benefit of the microscope is that it produces a better resolution than the naked eye. The higher…
Q: What is meant by the word sterile? Why is aseptic techniquenecessary for successful maintenance of…
A: Microbes are all pervading. They are found everywhere, in air, water, any substratum, inside and on…
Q: why is it important that you disinfect your work area both before and after working with bacteria?
A: Bacteria are present everywhere in each and every corner,present on non-living surfaces and as well…
Q: What is the purpose of using the streak plate method?
A: Solution- Introduction - Streak plate technique is used for the isolation into a pure culture of…
Q: Why did you perform the catalase test on colonies growing on nutrient agar plates but not on the…
A: Catalase test is used to identify the presence of catalase enzyme in the bacteria. Catalase is an…
Trending now
This is a popular solution!
Step by step
Solved in 3 steps
- What is the purpose of fixing a smear? Mark all that apply: To kill the bacteria so they aren't harmed by the staining method O To kill the bacteria to make the slide safer to handle O To attach the bacteria to the slide To break down the cell wall in order to make the cells accept stain To cause the cells to shrink and become distorted Microsoft Bing 11:30 AM do 87 F Sunny 9/14/2021Why is it important to limit the quantity of cells used to prepare a smear? Mark all that apply: 1. So that cells are not clumped and don't entrap stain creating erroneous results 2. So that the cells are spread out enough that cell morphology can be discerned 3. So that there are small groups of cells clumped together to make them visible 4. So that no contaminants are introduced onto the slide by being entrapped in clumps 5. So that the cells are spread out enough that the arrangement can be observedWhat is the purpose of fixing a smear? Mark all that apply: 1. To attach the bacteria to the slide 2. To cause the cells to shrink and become distorted 3. To kill the bacteria so they aren't harmed by the staining method 4. To break down the cell wall in order to make the cells accept stain 5. To kill the bacteria to make the slide safer to handle
- The benefits of the negative stain include: Mark all that apply: O No shrinkage or distortion of cells due to no heat or methanol fixing Because the cells don't take up stain they don't get shrunk or distorted O One can more accurately determine cell size and shape because the cells stand out against the background One can more accurately determine the cell size and shape because there is no shrinkage or distortion of cells O Negatively charged dyes are safer to work with than positively charged dyes IMicrosoft B 11:32 AHow does smear preparation of cells from a liquid medium differ from preparation of cells from a solid medium? Mark all that apply: O Water must be added to a smear from liquid media but not from solid O Water must be added to a smear made from solid media but not liquid media A smear made from liquid media will be more thick than a smear made from solid O A smear made from liquid media will be thinner than a smear made from solid media O A smear made from liquid media doesn't have to be fixed but a smear from solid media does Microsoft Bing 11:31 AM 87°F Sunny 9/14/2021I Why does't a negative stain colorize the cells in the smear?
- Which one of these techniques does not distinguish between live and dead cells? Group of answer choices Coulter count fluorescence staining spread plateSelect all that apply to a negative stain: 1. involves a washing step 2. cells may be distorted or shrunken 3. uses an acidic or negatively charged dye which stains the background 4. uses multiple dyes in the procedure 5. uses only 1 dye in the procedure 6. involves fixing 7. does not involve fixing 8. cells will not be distorted or shrunken 9. does not involve a washing step 10. can show cell morphology, size, and arrangement 11. uses a basic or positively charged dye which stains the bacterial cellsHow does smear preparation of cells from a liquid medium differ from preparation of cells from a solid medium? Mark all that apply: A smear made from liquid media will be thinner than a smear made from solid media A smear made from solid media doesn't have to be fixed but a smear from liquid media does A smear made from liquid media will be more thick than a smear made from solid Water must be added to a smear made from solid media but not liquid media A smear made from liquid media doesn't have to be fixed but a smear from solid media does Water must be added to a smear from liquid media but not from solid
- Principle/ technique used in Flagella Stain Method/ Preparation/ Procedure of Flagella StainBacterial smears are fixed before staining to O make their walls permeable. accept stain, affix the cells to the slide. O make the cells visible.A capsule stain is kind of: A Simple and Negative Stain B Differential and negative stain C Simple Stain D Differential stain E Negative Stain