Which of the following statements about genomic libraries and cDNA libraries is TRUE?O a genomic library contains exons onlyO a cDNA library is derived from chromosomal DNAO a genomic library is made using reverse transcriptaseO a genomic library is derived from mRNAO a cDNA library is derived from mRNA and is made using reverse transcriptase

A genomic library is a collection of DNA fragments that represents the entire genome of an organism.…

Which of the following statements about genomic libraries and cDNA libraries is TRUE? a genomic library contains exons only a cDNA library is derived from chromosomal DNA a genomic library is made using reverse transcriptase a genomic library is derived from mRNA O a cDNA library is derived from mRNA and is made using reverse transcriptase

Which of the following statements about genomic libraries and cDNA libraries is TRUE? a genomic library contains exons only a cDNA library is derived from chromosomal DNA a genomic library is made using reverse transcriptase a genomic library is derived from mRNA O a cDNA library is derived from mRNA and is made using reverse transcriptase

Human Heredity: Principles and Issues (MindTap Course List)

11th Edition

ISBN:9781305251052

Author:Michael Cummings

Publisher:Michael Cummings

Chapter9: Gene Expression And Gene Regulation

Section: Chapter Questions

Problem 17QP: Given the following mRNA, write the double-stranded DNA segment that served as the template....

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Which of the following apply to transcription: Synthesis of a chromosome using DNA polymerase that produces two new chromosomes O Synthesis of a DNA molecule using RNA polymerase that produces multiple genes O Synthesis of a mRNA molecule using DNA polymerase that produces protein O Synthesis of a mRNA molecule using RNA polymerase that is a copy of a gene O Synthesis of a protein from a specific mRNA molecule that requires a ribosome
The following image shows the 'beads-on-a-string' model of DNA. Where is Gene 1 located? Gene 1 Ac Ac (Ac (Ac O In the promoter DNA O In a nucleosome core particle O In the linker DNA O On a histone
A codon is a triplet of bases which codes for an amino acid. Exons are intervening sequence that are not spliced out during transcriptiona modification. RNA can never be double-stranded like DNA in both prokaryotes and eukaryotes. Alteration in BRCA-2 results to breast and ovarian cancer because of defect in the repair by homologous recombination. Write T if the statement is true and write F if the statement is false
Which of the following changes may cause a gene product to not loose functión? O Prevent or reduce transcription of the gene by deletion or alteration of the promoter O Delete or change the AUG translation initiation codon Repetitive sequence in the intronic region Disrupt the gene by a chromosomal rearrangement
RNA are extracted from liver cells and separated in agarose gel by electrophoresis side-by-side with a molecular weight marker. The separated RNA fragments are then transferred to an RNA-binding membrane. Next, this membrane is incubated with labelled probe specific for the gene X. This experiment determines: MOLECULAR BIOLOGY_basic how many copies of gene X there are in liver cells. if the gene X is translated in liver cells. if gene X has a point mutation in liver cells. the chromosomal location of gene X. the length of the transcript of gene X.
Cloning Genes Is a Multistep Process The following DNA sequence contains a six-base sequence that is a recognition and cutting site for a restriction enzyme. What is this sequence? Which enzyme will cut this sequence? (See Figure 13.5 for help.) 5 CCGAGGAAGCTTAC 3 3 GGCTCCTTCGAATG 5
Eukaryotic mRNA: usessnRNPs to cut out introns and seal together translatableexons. uses a spliceosome mechanism made of DNA to recognizeconsensus sequences to cut and splice. has a guanine cap on its 39 end and a poly(A) tail on its 59 end. is composed of adenine, thymine, guanine, and cytosine. codes the guanine cap and poly(A) tail from the DNAtemplate.
What Are Clones? Cloning is a general term used for whole organisms and DNA sequences. Define what we mean when we say we have a clone.
Given the following mRNA, write the double-stranded DNA segment that served as the template. Indicate both the 5 and the 3 ends of both DNA strands. Also write out the tRNA anticodons and the amino acid sequence of the protein encoded by the mRNA message. DNA: mRNA: 5-CCGCAUGUUCAGUGGGCGUAAACACUGA-3 protein: tRNA:
generated after a cDNA library is made unique sequences in the genome useful for mapping using sequence information all of the above
Which of the following best describes the process of DNA sequencing? a. DNA is separated on a gel, and the different bands are labeled with fluorescent nucleotides and scanned with a laser. b. A laser is used to fluorescently label the nucleotides present within the DNA, the DNA is run on a gel, and then the DNA is broken into fragments. c. Nucleotides are scanned with a laser and incorporated into the DNA that has been separated on a gel, and then the DNA is amplified with PCR. d. Fragments of DNA are produced in a reaction that labels them with any of four different fluorescent dyes, and the fragments then are run on a gel and scanned with a laser. e. DNA is broken down into its constituent nucleotides, and the nucleotides are then run on a gel and purified with a laser.
Which of the following features is common to both DNA replication and RNA transcription? Both RNA and DNA polymerase form a sugar-phosphate bond between the 3' hydroxyl and the 5' phosphate. Both RNA and DNA polymerase require oligonucleotide priming. Both RNA and DNA polymerase incorporate deoxyribonucleotides into the growing sequence. Both RNA and DNA polymerase initiate at promoter sequences. Both RNA and DNA polymerase have extensive proofreading editing.