Which of the following regarding the ATP Synthase is INCORRECT? Protons flow through the a and c subunits The translocation of four protons through the ATP synthase fuels the synt of one ATP molecule The F1 domain can function as an ATPase The y subunit rotates while the xß dimers are stationary
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- The standard free energy change for ATP hydrolysis is -7.3kcal/mol. The free energy change under typical cellular conditions is about -12kcal/mol. Why is there a difference? O The hydrolysis of ATP must be more difficult in the cellular environment O There is more ATP and less ADP in the cell O The cellular environment is anaerobic so O2 levels are reduced O The enzymes involved in the reaction are trapped in organellesV-class proton pumps run backward relative to the F-class ATP synthase. Consider the cartoon, which shows the conformations of the beta-subunits and ATPIADP + Pj of the F-class synthase. Which of the following associations between the conformation of the beta subunit and ATP/ADP + P¡ is correct for V- Binding Change Mechanism loose binding ADP+P ATP ATP class pumps? C repeat ADP + P, ADP АТР tight binding АТР +P оpen АТР O The open conformation releases ATP. Hydrolysis of ATP to ADP + P¡ drives the change from tight to loose. O Binding of ADP + P¡ drives change from open to loose. Hydrolysis of ATP to ADP + Pj drives the change from open to loose.Which is the correct link between conformation of the beta-subunit of the F1 component of the ATP synthase and ADP+P¡ or ATP? loose Binding Change Mechanism binding ATP ADP+P ATP Crepeat ADP ADP +P; ATP ATP +P; tight binding open ATP loose => binding ADP+Pi open => catalyzes ADP+P¡ into ATP tight => releases ATP open => releases ADP+P¡
- Whatis the main idea behind the conformational coupling mechanism for ATP synthase? Describe the three conformational states.Which of the following statements about oxidative phosphorylation is correct? O H+ ions are transferred from Complex I or Complex II to ATP synthase where ATP production occ O Proton pumps transfer electrons from the cytosol to the mitochondrial matrix as electrons are tra O The chemical and electrical gradient is established between the intermembrane space and the ma electron carriers. O ATP synthase pumps electrons back to the intermembrane space as a consequence of electrocher mitochondrial matrix. • PreviousIf actively respiring mitochondria are exposed to an inhibitor of ATPADP translocase, the electrontransport chain ceases to operate. Why?
- Propose that you have discovered a new ATP synthase from the mitochondrion of an organism onthe planet X. You found that the mitochondrion from this newly discovered organism has exactly thesame electron transport chain as human beings. In addition, this newly discovered ATP synthase hassimilar subunit composition and arrangements as human beings, excepting that such newlydiscovered ATP synthase contain 6 c-subunits. Theoretically, the P/O ratio for the oxidation of FADH2 to water in this organism is: 1 1.5 2 2.5 3 3.5 None of the above Need more information Which statement is NOT true about the transport of ATP across the inner mitochondrial membraneon its way to the cytosol? The transport causes the loss of a net charge of -1 in the matrix. It is accomplished by adenine nucleotide translocase. The same enzyme that transports ATP also transport ADP in the opposite direction. It is complexed with Mg2+ to reduce the draw on the electrical part of the protonmotive force.Two biochemistry students are about to usemitochondria isolated from rat liver for an experiment on oxidative phosphorylation. The directions for the experiment specifyaddition of purified cytochrome c from any source to the reactionmixture. Why is the added cytochrome c needed? Why does thesource not have to be the same as that of the mitochondria?20.12 Classify the enzyme that catalyzes each of the following reactions: |3| a. CH3-C-COO + CO2 + ATP OOC-CH2-C-COO + ADP + P; + H b. CH3-CH2-OH + NAD* CH3-C-H + NADH + H*
- Medical researchers are studying a patient with the newly discovered u LEKS metabolic syndrome, in which a genetic mutaton prevents NADH produced in the cytosol from being reoxidized by the electron transport chain. Suppose the following nutrient molecule is digested and metabolized by this patient under anaerobic conditions, e.g. during a sudden intense exertion: de HO-CH: -OH Он н. HO H OH How many ATP molecules will uttimately be produced by the catabolism of this molecule? Note: you can ussume the catabohism is as complete as it can be under the conditions given above. How many molecules of pyruvate will be produced during dycalysist How many maleoules of lactate will be producedThe standard free energy of hydrolysis of inorganic polyphosphate (polyP) is about −20 kJ/mol for each Pi released. We calculated in Worked Example 13–2 that, in a cell, it takes about 50 kJ/mol of energy to synthesize ATPfrom ADP and Pi. Is it feasible for a cell to use polyphosphate to synthesize ATP from ADP? Explain your answer.Cyanide poisoning inhibits aerobic respiration at cytochrome c oxidase. Which of the following is NOT a result of cyanide poisoning at the cellular level? Select an answer and submit. For keyboard navigation, use the up/down arrow keys to select an answer. a b с d e Oxygen is reduced to water The rate of glycolysis increases Cells are forced to switch to anaerobic respiration The electron transport chain is not completed None of the above Answered K Open in Reading View ✔Posubmit