Which of the following is not a reason we often perform metagenomics studies by doing PCR using long degenerate primers for the 16S gene? Eliminates issue of having to randomly fragment DNA for sequencing (which can sometimes be tricky to optimize) All microorganisms have a 16S gene It is taxonomically informative and allows you to identify taxa using a database You can estimate relative taxa abundance by relative read abundance, avoiding genome size bias. You can make a phylogeny allowing you to do diversity analyses 16S has conserved regions and the primers used allow for some sequence variation, so we can amplify the region from different taxa efficiently using PCR

The 16S rRNA (ribosomal ribonucleic acid) of prokaryotes is used for performing metagenomic studies.…

Answered: Which of the following is not a reason…

Human Heredity: Principles and Issues (MindTap Course List)

11th Edition

ISBN:9781305251052

Author:Michael Cummings

Publisher:Michael Cummings

Chapter15: Genomes And Genomics

Section: Chapter Questions

Problem 7QP: Which of the following is NOT an activity carried out in the field of bioinformatics? a. collecting...

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Which of the following is not a reason we often perform metagenomics studies by doing PCR using long degenerate primers for the 16S gene?

		Eliminates issue of having to randomly fragment DNA for sequencing (which can sometimes be tricky to optimize)
		All microorganisms have a 16S gene
		It is taxonomically informative and allows you to identify taxa using a database
		You can estimate relative taxa abundance by relative read abundance, avoiding genome size bias.
		You can make a phylogeny allowing you to do diversity analyses
		16S has conserved regions and the primers used allow for some sequence variation, so we can amplify the region from different taxa efficiently using PCR

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