Which of the following is a measure of CPE from a viral infection? 1. End point dilution assay 2. ELISA 3. Plaque Assay 4. Hemagglutination assay 5. 1 & 3
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Which of the following is a measure of CPE from a viral infection?
1. End point dilution assay 2. ELISA 3. Plaque Assay 4. Hemagglutination assay 5. 1 & 3
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- Write a lab report on enumeration of lytic viruses : the plaque assay including the sections: introduction, Materials and Methods, Results and Discussion, and citing peer reviewed sources use this data for results section Group Number Number of Plaques Dilution Factor of Plate Original Concentration of Phage 1 58 10^3 5.80E+04 2 37 10^3 3.70E+04 3 44 10^3 4.40E+04 4 63 10^3 6.30E+04 5 36 10^2 3.60E+03 6 65 10^3 6.50E+04 7 260 10^2 2.60E+04 8 39 10^3 3.90E+04 9 178 10^2 1.78E+04 10 192 10^2 1.92E+04 11 274 10^2 2.74E+04 12 224 10^2 2.24E+04 Class Average 3.52E+04Why is the ELISA test used to screen human serum for the AIDSvirus, while the Western blot procedure is used only as the confirmation test?A plaque assay is an effective way to determine the concentration of lysogenic phage in a solution. A. True B. False
- Discuss the difference between the nontreponemal agglutination test and the treponemal agglutination test. pls make it organize and if possible do it in table form to really show the difference, but if not just make it comprehensive and detailWhy are agglutination tests so widely used in clinicaldiagnostics? How are fluorescent antibodies used todiagnose diseases? What advantages do immunofluorescenttechniques have over traditional culturing?Figure 1. Cytotoxicity test on S96 well plates that was monitored by ELISA reader 3- Discussion 1- Explain another methods by which cytotoxicity is measured ?
- You do a viral plaque assay using the six well plate shown below. The dilution scheme, what was infected on each well, and the results of the plaque assay are shown below. What is the viral titer of the original lysate? A. ~1.1 *10^6 PFU/mL B. ~1 *10^7 PFU/mL C. ~3 * 10^7 PFU/mL D. ~2 * 10^6 PFU/mL E. ~3.5 *10^6 PFU/mL F. ~1.1 * 10^5 PFU/mL G. ~3.5 *10^5 PFU/mLWhat are the available dosage forms of:21. Inoflox®22. Isoket®23. Isoxilan®24. Kefox®25. Purinase®29. Correctly label each image below with the ELISA method it is describing (3pts) Sand Ag Substrate ghta feel 30SICA S Primary Antibody Conjugate Substrate Secondary Antibody Conjugate You Substrate Capture Antibody Seeslo aidi onirif gnil 30. Andrew is a doctor in a hospital. He is treating a patient with a bacterial respiratory infection. He wants to know what the best antibiotic to prescribe for this infection is, so isolates the microorganism and does a Kirby-Bauer test. His results are pictured below. (3pts)
- If you wanted to make an assay detecting SARS-CoV-2 virus presence, like the assay shown in the previous question, what would be a logical combination of things to include in your list of supplies to obtain? rabbit anti-SARS-cov2 spike antibody, anti-rabbit IgG horseradish peroxidase-conjugated antibody, tetramethylbenzidine rabbit anti-SARS-cov2 spike antibody, anti-mouse horseradish peroxidase conjugated antibody, tetramethylbenzidine rabbit anti-SARS-cov2 spike antibody, anti-rabbit SARS-cov2 spike antibody, tetramethylbenzidine SARS-cov2 spike, anti-rabbit IgG horseradish peroxidase-conjugated antibody, tetramethylbenzidine rabbit anti-SARS-cov2 spike horseradish peroxidase conjugated antibody, anti-rabbit horseradish peroxidase conjugated antibody, tetramethylbenzidineOutline the principle behind the following techniques: 1. Immunofixation 2. Southern blotting 3. Ion exchange chromatograph 4. SDS-PAGE.How is RT PCR used to detect Ebola? How does the actual RT PCR procedure work? How is ELISA used to detect Ebola? What is the method? What binds to what? How is Immunohistochemistry (IHC) used to detect Ebola? What is the method? What tissue is used, what binds to what?