When analyzing a PTH (phenylthiohydratoin) derivative of the amino acid isoleucine, resulting from one round of an Edman Degradation experiment, which of the following experimental reverse phase chromatography results would you expect to see? a.)The isoleucine-PTH derivative will elute from the hydrophobic column with a greater retention time relative to most other amino acid-PTH derivatives because it is relatively non polar. b.)The isoleucine-ÞTH derivative will elute from the hydrophobic column with a lesser retention time relative to most other amino acid-PTH adducts because it is relatively polar. c.)The isoleucine-PTH derivative will elute from the hydrophobic column with a retention time in between the groups of polar and non polar amino acid-PTH derivatives because it is neither polar nor non polar.
When analyzing a PTH (phenylthiohydratoin) derivative of the amino acid isoleucine, resulting from one round of an Edman Degradation experiment, which of the following experimental reverse phase chromatography results would you expect to see? a.)The isoleucine-PTH derivative will elute from the hydrophobic column with a greater retention time relative to most other amino acid-PTH derivatives because it is relatively non polar. b.)The isoleucine-ÞTH derivative will elute from the hydrophobic column with a lesser retention time relative to most other amino acid-PTH adducts because it is relatively polar. c.)The isoleucine-PTH derivative will elute from the hydrophobic column with a retention time in between the groups of polar and non polar amino acid-PTH derivatives because it is neither polar nor non polar.
Chapter34: Miscellaneous Separation Methods
Section: Chapter Questions
Problem 34.10QAP
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