When a mixture of DNA fragments of different sizes is loaded onto an agarose gel and a voltage is applied, the DNA will migrate in the gel. Which molecules will migrate the fastest? O The molecules with the greatest fraction of A/T nucleotides The shortest The molecules with the greatest fraction of G/C nucleotides O The longest

When a mixture of DNA fragments of different sizes is loaded onto an agarose gel and a voltage is applied, the DNA will migrate in the gel. Which molecules will migrate the fastest? O The molecules with the greatest fraction of A/T nucleotides The shortest The molecules with the greatest fraction of G/C nucleotides O The longest

Human Heredity: Principles and Issues (MindTap Course List)

11th Edition

ISBN:9781305251052

Author:Michael Cummings

Publisher:Michael Cummings

Chapter8: The Structure, Replication, And Chromosomal Organization Of Dna

Section: Chapter Questions

Problem 15QP: Using Figures 8.7 and 8.9 as a guide, draw a dinucleotide composed of C and A. Next to this, draw...

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The two strands for the helix-shaped DNA molecule are heldtogether by electrostatic forces as shown in class. Due to electron sharing, themagnitude of the net average charge on the H and N atoms, each, is 0.2e. Thenet average charge on the C and O atoms, each, is 0.4e. The distance betweenany two adjacent atoms on the same molecule is 0.1nm. Since electric fieldsdecrease as an inverse square law, they die off quickly with distance. So, theonly fields that really contribute to any given bond are due to the 3 atomsalong the red dotted line in the class diagram (two atoms on one'molecule andone atom on the other molecule).Calculate the electric fields and net force between a thymineand an adenine pair.=Calculate the electric fields and net force between a cytosineand a guanine pair.nConsider the OHN bond in the AT nucleotide. Can theelectric field be zero anywhere along the axis of this bond? If not,explain why not. If so, find where the electric field is zero.
Which of the following changes occur with a double-stranded DNA if it is heated to 95 C at neutral pH? OA Increase in the absorption of uitraviolet light at 260 nm OB. Unwinding of the helical DNA structure Oc Separation of the two DNA strands Do.Hydrolysis of the N-glycosidic bond belween the base and deoxyribose DE Breakage of the phosphodiester bonds belwean adjacent ucieotides Dr. Breakage of the hydrogen bonds between guanines and cytidines.
How many times wider is a 30 nm fiber than a DNA double helix? Show your work.
Which of the following DNA sequences will travel the shortest amount down a gel? A ATTGCAGGCCCT GCGATGCTAGC TTACGATCGGAA D GCTACTGATС
Given the active site diagram below, please identify the residue participating in a charge-charge interation. HO OH HN OH 5 ΝΗ *HN ΝΗ OH O Zn²+ 2 4 5 3 1 2 NH 3
In DNA and plasmid extraction using the kit method, silica membrane is used. How could the nucleic acid attach to the membrane? O by phosphodiester bonding to the silica matrix O by hydrogen bonding in high salt conditions O by covalent bonding between nucleic acid and silica O by hydrophobic bonding in high chaotrophic salt conditions
A piece of DNA is cut into four fragments as shown below. A solution containing the four fragments is placed in a single well at the top of an agarose gel. Using the information given below, draw (below the well) how you think the fragments will be aligned on the gell following electrophoresis. Label each fragment with its corresponding letter. Each band on the gel will be the same width, equal to the width of the well at the top of the gell. These should be in one lane. Do smaller or larger (pb) fragments migrate the furthest? Why?
The joining of a new nucleotide to a growing strand during DNA synthesis leads to the formation of a phosphoester bond and involves O a nucleophilic attack of the terminal P atom of the growing strand on the electrophilic 3-0 atom of the growing strand O a nucleophilic attack of the terminal 3'-0 atom of the growing strand on the electrophilic P atom of the incoming nucleotide O an electrophilic attack of the terminal 3'-O atom of the growing strand on the nucleophilic P atom of the incoming nucleotide an electrophilic attack of the terminal Patom of the growing strand on the nucleophilic 3-O atom of the growing strand
In the following diagram of DNA replication fork, A is A e 5' 3" 3'OH 51 3 OH 5'
What is the purpose of using the Chelex beads while extracting the DNA at high temperature from your cheek cells? Group of answer choices The Chelex binds cations like Mg2+ that are co-factors for nuclease enzymes which would otherwise degrade the DNA. The Chelex denatures the DNA and makes the DNA single stranded. The Chelex will bind the DNA tightly after the cells are ruptured, helping extract it from the cells. The Chelex provides Mg2+ co-factors needed by DNA polymerase enzymes to make more copies of DNA
DNA fragments can be separated by: O gel electrophoresis O autoradiography O x-ray crystallography O centrifugation
Examine the 5'- 3 sequence of bases of the DNA molecules (A D) shown below. I am only showing you the 5 - 3' strand of each molecule, but you can imagine the complementary 3' - 5' strand for each molecule. Which double-stranded molecule is held together by 10 hydrogen bonds? O AAAT O ATAG O TGTC O GCGA