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- Transmittance (%) 100% 75% 50% 25% 0% 4000 3500 3000 OH 2500 2000 Spectrum 3B 1750 حسيمة 1500 Wavenumber (cm-¹) 1250 .OH 1000 OH 750 5000.8 0.6 0.4 0.2 0.0 3000 2000 1000 Wavenumber (cm-1) ОН NH2 `NH2 A В C TransmitancePotassium dichromate and potassium permanganate have overlapping absorptionspectra in 1M H2SO4 . K2Cr2O7 has an absorption maxima at 440 nm, and KMnO 4 has a band at 545nm (the maximum is actually at 525 nm, but the longer wavelength is generally used whereinterference from K2C2O7 is less). A mixture is analyzed by measuring the absorbance at these twowavelengths with the following results: A 440 =0.405, A 545 =0.712 in a 1-cm cell. The absorbances ofpure solutions of K2Cr2O7 (1x10 -3 M) and KMnO 4 (2.00x10 -4 M) in 1 M H 2 SO 4 , using the same cell gavethe following results: A Cr,440 = 0.374, A Cr,545 = 0.009, A Mn,440 =0.019, A Mn,545 = 0.475. Calculate theconcentrations of dichromate and permanganate in the sample solution.
- 3.) The accuracy of a spectrophotometer is evaluated by preparing a solution of 60.06 ppm K2Cr2O7 and measuring its absorbance at a wavelength of 350 nm in a cell with a pathlength of 1.00 cm. The expected absorbance is 0.640. What is the expected molar absorptivity of K2Cr2O7 at this wavelength? 4.) A solution’s transmittance is 35.0%. What is the transmittance if you dilute the solution to half of its initial concentration?100 - 80 60 40 20 10 15 20 25 30 35 40 45 50 55 60 m/z Absorbance / % 50- 100+ 4000 3000 2500 2000 1500 1000 500 Wavenumbers / cm Relative Intensity / %You will check the outcome of reaction via infra-red spectroscopy: what are the diagnostic functional group peaks for the following compounds? You should also indicate the stretching motions responsible for absorbance. Dilantin benzil (unreacted starting material) Urea (unreacted starting material)
- QUESTION 5 The IR, 'H NMR and 1°C NMR spectra of compound P are shown below. The molecular formula for compound P is CsH;NO2. Identify three (3) important absorption peaks on the IR spectrum and indicate the bond and wavenumber associated with each peak. Elucidate the structure of compound P and assign each peak in the 'H and 13C NMR spectra. (Hint: The compound consists of more than one functional group). а. b.Express the following absorbances (A) in terms of transmittance (T) and percent transmittance (%T) I. Note that: T- 10; %T-Tx100 A %T 1. 0.052 0.916 0.378 0.269 0.075 2345Describe the origin of shot noise in a spectrophotometer. How does the relativeuncertainty vary with concentration if shot noise is the major noise source?
- 6. C8H100 11 TRANSMITTANCE LOD HSP-04-492 0+ 10 4000 9 3000 8 5 7 6 : 5 ppm 2000 4 HAVENUMBERI-l 2: 2 1 3 1500 2 سهر 1 1000 0 500 200 T 180 CDS-06-431 DEPT-90 T 160 DEPT-135 140 120 100 لك -2 peaks u ppm 80 60 40 20 0. What is the molar absorptivity when the percent transmittance is 56.2%, the pathlength is 2.00 cm, and the concentration is 0.0000748 M?Lrdocaine USP (ATR) 95- 90 85 80 75 70 65 60 55 50 45 Lidocaine 40 4000 3500 3000 2500 2000 1500 1000 Wavenumbers (cm-1) %Transmittance IZ 3243.36 3021.30 2968.47 2921.13 2799.39 1661.66 1593.98 1491.43, 1449.061 422.12,384.681371.62 1291.03264.55 1206.99 1165.931119.56 1091.78072.77. 1034.33 970.79986.39 917.91, 885.23 763.76 703.94 615.01599.90