What modifications must you do to your samples or calibration curve so that you can accurately measure the absorbance and calculate the correct calcium concentrations for all samples
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What modifications must you do to your samples or calibration curve so that you can accurately measure the absorbance and calculate the correct calcium concentrations for all samples
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- The nurse notes the patient's IV pump is set at 12 mL/hr. The IV bag holds 20 units of Pitocin mixed in 500. mL of Lactated Ringers. How many mu/min is the patient receiving. Note that mu is milliunits. 6mu/min 48 mu/min 12 mu/min 0.48 mu/min 8 mu/minThe BSA stock solution from the previous problem was then diluted to generate a set of standard solutions of known concentrations. After performing biuret assay on these solutions, their absorbance at 540 nm were measured using a UV-vis spectrophotometer. The following data were obtained. Concetration of BSA Absorbance (mg/mL) 0.1 0.048 0.2 0.095 0.4 0.191 0.6 0.290 0.8 0.380 1.0 0.485 Calculate the (a) Linearity constant (r), (b) y-intercept, (c) Slope, and (d) Protein content of an unknown sample having an absorbance of 0.325.Does measuring using higher (50mL vs 100mL) produce more accurate and precise results? Why?
- Assume your field of view under a 3X objective is 6mm across. What is the field of view in mm under a 20X objective? Show clear work with units for credit. Field of view 20X objective? ___mm What is the field of view of this 20X objective in micrometers? If you observe a specimen under the 20X objective and the specimen spans one-quarter of the field of view distance, how long is the specimen in micrometers?Which of the following is/are source/s of error in performing a spectrophotometric method? Answer all that apply. Sample is turbid when the absorbance is read. The absorbances were read at maximum absorption. Standard solutions are prepared accurately. The cuvettes have fingerprints.Which of the following statements are true about the use of a blank in spectrophotometry? Select all that apply. Select 2 correct answer(s) A blank is needed because the spectrophotometer also measures the absorbance of the cuvette and water. A blank must be measured prior to measuring your sample. None of the answer choices listed are correct. A blank must be measured after measuring your sample. A blank is only needed when the concentration of your sample is known.
- Which spectrophotometer mode should you use to calibrate the instrument and which mode should you use to read your samples? Transmittance for both Absorbance for both Absorbance to calibrate and transmittance to read samples Transmittance to calibrate and absorbance to read samples Flip a coin to pick a mode each time.Which of the following scientists use this X-ray crystallography?Which of the ff. is carried out when sample has a low absorbance? Use a cuvette with shorter width. Use a cuvette with longer width. Use a quartz cuvette instead of ordinary glass. O Use ordinary glass cuvette instead of quartz.
- What will you do if you see bubbles forming on the gel after pouring it onto the gel caster/tray?The reults for the macroscopic part: 0.30M glycerin – solution was translucent (could see text behind the test tube) 0.15M NaCl – solution was opaque (could not see text behind the test tube) 0.30M NaCl – solution was opaque (could not see text behind the test tube) 0.15M glucose – solution was translucent (could see text behind the test tube) 0.30M glucose – solution was opaque (could not see text behind the test tube) 0.30M Urea – solution was translucent (could see text behind the test tube) Results for microscopic part: 0.30M glycerin – no cells present 0.15M NaCl – normal sized cells 0.30M NaCl – crenated (shrunken and star-shaped) cells 0.15M glucose – no cells present 0.30M glucose – normal sized cells 0.30M Urea – no cells present Determine the osmolarity (hypoosmotic, isosmotic, or hyperosmotic) and tonicity (hypotonic, isotonic, hypertonic) of the following solutions.In which solutions did the osmolarity NOT match the tonicity? For those solutions, why did the osmolarity…Put the following steps for running a gel in order. first Remove comb and put gel int v [ Choose ] Load samples into gel Let gel solidify for 15-20 minutes Connect electrodes and turn on power to run gel second Pour heated, liquid agarose into gel cartridge Remove comb and put gel into bottom of electrophoresis box Pour buffer into electrophoresis box to cover gel third fourth Pour heated, liquid agarose in v fifth Load samples into gel sixth Connect electrodes and turn v