What is the Rf value of each amino acid observed? What are the possible components of the amino acid mixture? Answer can be up to two amino acids per component.
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- What is the Rf value of each amino acid observed?
- What are the possible components of the amino acid mixture? Answer can be up to two amino acids per component.
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- The standard curve to determine the amount of betacyanin is shown below. You extracted a red pigment from a beet disc (the mass of a disc is 2 g) using 10 ml of 20% ethanol solution. You measured absorbance of the solution above the beet disc every minute for a total time of 20 minutes. The increase in absorbance was linear during a period of time from 1 min to 10 min. The absorbance at 10 min was 0.8. Calculate the amount of betacyanin extracted from 1g of a beet tissue per minute. Explain your calculations. You can use Excel or a calculator.A 5% dextrose in 1/2 normal saline (D5 1/2 NS) solution is commonly administered to patients needing post-operative IV fluids. How could you prepare 500 mL of this solution? Dextrose is 5% (m/v) and the NaCl is 0.45% (m/v).a) What are the methods used for cholesterol determination? Explain the basis of the method you used in your experiment by writing.b) In the experiment conducted for the determination of cholesterol, the function of the calibration curve obtained using standard cholesterol solutions of 25-400mg/dl was determined as y= 0.0009x.Since the absorbance of the serum sample was determined as 0.28, how many mg/L is the amount of cholesterol in the sample
- The doctor ordered Oxacillin 650mg every 6 hours. The instructions on the 2gm vial states to reconstitute with powder with 11.5ml of sterile water for injection, yielding 220mg/1.5ml. How many ml will you administer? using dimensional analysis and show workA 2.5mg/ml solution of ampicillin is available for use, what volume would youadd to 200ml of LB medium to obtain a final concentration of 50µg/ml ofampicillin?Solution Absorbance mg/ml aspirin Standard solution - 1.6 mg/mL A 0.638 0.08 mg/mL B 0.504 0.064 mg/mL C 0.376 0.048 mg/mL D 0.259 0.032 mg/mL E 0.126 0.016 mg/mL A = -log T where T = %T ÷ 100 Construct a callibration curve using the above data. Absorbance should be on the vertical axis and "mg/mL of acetylsalicylic acid" on the horizontal axis. The line should go through the origin. Using the data provided, the graph you have generated, and the procedure that was used to generate the solutions which were examined by spectroscopy, calculate the amount of acetylsalicylic acid per tablet. Commercial tablet 1 labelled as 100 mg enteric coated Absorbance = 0.16 Commercial tablet 2 labelled as 300 mg Absorbance = 0.45 Student prepared tablet from practical 5 Absorbance = 0.19 Using the data provided, the graph you have generated, and the procedure that was used…
- The calibration curve shown below was used to analyze an unknown protein solution. What is the concentration of the unknown solution, if the absorbance of the unknown is 0.505? Answer in ug/mL. 1.2 y%3D0.005x+0.061 R=0.992 0.8 0.6 0.4 0.2 50 100 150 200 250 Concentration ug/ml AbsorbanceFour known amino acids (AA) produce a chromatograph with Rf values as follows: AA1 =0.25, AA2 =0.66, AA3=0.50,AA4=0.80. A mixture of three amino acids yields spots that are 2 cm,5.2cm, and 6.4 cm from the organ. The solvent front is 8.0 cm from the origin. Which three of the four amino acids are contained in the mixture?If a pharmacist needs to prepare a bacterial suspension with a concentration of 2.4x10^2 cfu using 1 mL of 2.4 x 10^4 bacterial suspension, what size of volumetric flask will she/he be needing?
- A glucosamine solution of unknown concentration was diluted as follows: 1 mL of the solution was diluted to 5.0 mL. A 0.3 mL aliquot of this solution was diluted with 0.7 mL of water; the absorbance of this was measured and found to be 0.440 (uncorrected, must subtract blank). What is the concentration of the original undiluted glucosamine hydrochloride solution in µmol/mL? Given: Corrected absorbance = 0.395 y= 1.165x+0.055 Standard equationThe results observed in a patient admitted to an emergency department with confusion and oliguria were reviewed (reference ranges are given in brackets): Sodium Potassium Urea Creatinine Alkaline phosphatase Alanine aminotransferase Albumin Bilirubin Calcium 146 mmol/L 7.2 mmol/L 18.5 mmol/L 256 μmol/L 319 IU/L 32 IU/L 42 g/L 13 μmol/L 2.47 mmol/L (135-145) (3.5-5.0) (3.5-6.6) (70-150) (95-320) (a) What is the electrolyte abnormality in this patient? (b) What do the data suggest? (c) What action should you take? (5-42) (35-50) (<17) (2.12-2.62) The sample was not visibly haemolysed and was centrifuged and analysed in the labora- tory within one hour of being collected.A mixture containing only KCl and NaBr is analyzed by the Mohr Method. A 0.3172-g sample is dissolved in 50 mL water and titrated to the Ag2CrO4 endpoint, requiring 36.85 mL of 0.1120 M AgNO3. A blank titration requires 0.71 mL of titrant to reach the same endpoint. Report the % (w/w) KCl and NaBr in the sample. [Ans . 84.41 % (w /w); 17.59 % (w /w)]