What error is introduced into the determination of an Rf value if the top is left off the developing chamber?

Q: Increasing reagent concentration makes the change in pAg larger.

A: we know, pAg =  -log[Ag+]. so with increasing the concentration of reagents,pAg decreases. Option C…

Q: A student spots an unknown sample on a TLC plate and develops it in pentane solvent. Only one spot,…

A: The retardation factor (Rf) provides the interaction of the substance with the thin layer of the…

Q: How do i know my starting material was fully consumed using a TLC plate comparing the raction…

A: TLC means thin layer chromatography is a chromatographic technique used for the separation of…

Q: 9) If after drying, the TLC plate showed spot A showed a Rf of 0.20 and spot B showed a Rf of 0.22.…

A: During the detection and separation of components in a compound using TLC, the solvent front is kept…

Q: Which of the following results from an in-line HPLC mobile phase filter becoming plugged with…

A: Correct option is B.

Q: How does it affect the peak produced in gas chromatography when: – Inserting samples is too slow –…

A: ✓Chromatography is a technique that separates components in a mixture by the difference in…

Q: What is the Rf value for the uppermost yellow spot in TLC plate 3 (plate imaged below)? It is…

A: The TLC plate 3 with all the spots is given below :

Q: Why line extraction from a point cloud is needed ?

A: Planar structure extraction from point clouds is an important process in many applications.

Q: It's important to mark the solvent front with a pencil after the plate is finished being developed…

A: It's important to mark the solvent front with a pencil after the plate is finished being developed…

Q: Indicate which other analytical techniques TGA can be coupled with and what would be the advantages?

A:

Q: What is the Rf value for a compound if it moves 3.0 cm from the origin when the initial spot is…

A:

Q: Report the R value of the best-fit line for the Calibration Curve

A: R-squared is a statistical measure of how close the data are to the fitted regression line. It is…

Q: It is never necessary to take more than one composite sample per field. True Or False

A: Composite soil sampling is the traditional soil sampling method. To make a composite sample we mix…

Q: Can you DRAW a TLC plate in an elution chamber that shows 3 lanes each with one spot. Label the…

A: A thin layer chromatography (TLC) is used to seperate components of mixture using thin stationery…

Q: If a spot travels 2.3 cm while the mobile phase travels 6.6 cm, what is the Rf value of the…

A: Given: Distance travelled by spot = 2.3 cm Distance travelled by mobile phase = 6.6 cm

Q: In reverse phase chromatography which analyte will elute first, most polar or least polar?

A: In reverse phase chromatography, polar solvent is used as a mobile phase.

Q: Explain how the charged aerosol detector used

A: Charged aerosol detector: The detector, which is used to detect (measure) the amount of charged…

Q: Why should you not use a ballpoint pen when marking a TLC plate and why does the horizontal line…

A: If ball point pen is used, the ink will travel up with the solvent, just like the samples.  The…

Q: Why is splitless injection used with purge and trap sample preparation?

A: The collection of all of the analyte from the unknown and to inject whole of the analyte into the…

Q: Explain how solid-phase microextraction works. Why is cold trapping necessary during injection with…

A:

Q: Which is more polar, the water solvent or the chromatogrphy paper?

A: In paper chromatography, paper is made up of cellulose in which there are various hydroxyl group…

Q: What are the possible reasons why there are no spots on the Developed Plate after analysis?

A:

Q: How does temperature, flow rate of carrier gas, boiling point and column suupport affect gas…

A: Explanation. Retention time-     Retention time is the amount of time a compound spends on…

Q: if air can degrade a column and cause TC detector filaments to burn out, why do we use an air peak?

A: Interpretation -  If air can degrade a column and cause TC detector filaments to burn out, why do we…

Q: Why is it not necessary to test the supernatant from both trial samples for the excess reactant is…

A: In step 9, BaCl2 is added to test tube 1, and Na3PO4 is added to test tube 2. Precipitation occurs…

Q: The eluent is to be below the 1.5 cm line on the chromatographic paper. Describe the expected…

A: Introduction- Thin layer chromatography(TLC) is a separation technique where the compounds are…

Q: Which type of gas chromatographic column consists of a tube that is densely packed with uniform,…

A: An analytical technique which is used to separate components present in a mixture and to determine…

Q: What would be the effect of increasing the carrier gas flow rate on the retention rate?

A: Retention time is the distance that the compound cover from the time of injection till the maxima in…

Q: Can you TYPE the definitions of the all of words please Chromatography TLC Stationary phase Mobile…

A: Most of the time, the compounds we encounter are not in their purest form. They are essentially a…

Q: At what stage was the internal standard added to the prepartions, in the protocol of this…

A: Step : 1             The required answer for the above question is follows as :

Q: Investigate the linear calibration range of this experiment.

A: Lead Concentration, ng ml-1 Absorbance 10 0.05 25 0.17 50 0.32 100 0.6 200 1.07…

Q: a pigment in the thin layer chromatography traveled beyond or farther than the solvent front.…

A: As a pigment in the thin layer chromatography traveled beyond or farther the solvent front therefore…

Q: An orange compound was added to the top of a chromatography column. Solvent was added immediately,…

A: An orange compound was added to the top of a chromatography column. The solvent was added…

Q: Why should the syringe be removed from the sensor when setting the initial volume for each trial?

A: Option C

Q: Why should you store the crucible plus sample in a desiccator after heating and before weighing?

A: A crucible is a ceramic container similar shallow cup that is used to heat substance. If the…

Q: Peak Retention Time (minutes) Area 1.201 136501204 acetone 2.011 1253056 toluene ortho-product…

A:

Q: 2-naphthol IR Tables that contains the important peaks for the experimental

A:

Q: Substance C is made up of components A and B. A sample of C was dotted on the TLC plate below. Using…

A:

Q: 1. a) "Solution 1" is made from the addition of water to a portion of the 0.0500 M  Fe3+ "…

A: Given, Molarity of prototype solution = 0.05M Fe3+. Volume of prototype solution = 10.0mL. Volume of…

Q: What are possible sources of loss in an extraction experiment?

A: Extraction is a kind of fundamental technique which is used to isolate one compound from a given…

Q: What property determines which layer is on top or bottom in an extraction?

A: Since you have asked multiple question, we will solve the first question for you. If you want any…

Q: A student spots an unknown sample on a TLC plate. A single spot with an Rf of 0.55 showed up on the…

A:

Q: what will produce a smaller Rf value if the solvent and paper are both changed?

A: Increasing the polarity of solvent will result in increase in Rf value as the compound in even for…

Q: Why must the spots be above the level of the development solvent in the developing chamber?

A: Given statement,     The spots be above the level of the development solvent in the developing…

Q: If peak A has a width of 10 min and peak B has a width of 16 mins, what is the column resolution?

A: Given :-  Width of peak A = WA = 10 min Width of peak B = WB = 16 min  Column resolution = ?…

Q: A technician injects a sample twice back to back into a C18 General Purpose HP column. Sample…

A: One extra peak in first injection may be due to the presence of contamination from septum, injection…

Q: How GC retention time can be affected by the mobile phase flow rate and the temperature of the…

A:

Q: The following conditions may not cause tailing EXCEPT a. Thick filter paper as the stationary phase…

A: Tailing in thin layer chromatography :  Due to the using high concentration of solute or other…

Q: Does the amount of radiation (cpm) increase or decrease as the distance between the sorce and the…

A: Amount of radiation between the source and sensor is determine using the inverse square formula:…