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The molar absorptivity of Allura Red is 2.59 x 104 M-1 cm-1, account for the accuracy of the modified set-up. What could be the factors that contribute to the inaccuracy or the modified spectrophotometric set-up?
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- In the food dye experiment, the molar absorptivity of Allura Red is 2.59 x 104 M-1cm-1, account for factors that may affect the accuracy of the modified spectrophotometric set up?What could be the factors that contribute to the inaccuracy of the modified spectrophotometric set-up?1. For determining the %w/w of salicylamide and caffeine in an unknown sample using acetaminophen as an internal standard using a HPLC a) Is it possible to use the λmax (273 nm) of caffeine in the experiment instead of using 254 nm? Explain your reasoning.b) What are some advantages of using the Internal Standard method for quantitation over the External Standard method?c) How is the signal intensity seen in the chromatogram affected by the wavelength used for analysis?
- What are the similarities between fluorescence detectors in HPLC and chemiluminescence detectors in HPLC?Explain the need for an internal standard in quantitative NMR AND indicate which properties are desirable in an internal standard to be used in quantitative13. Electrospray time-of-flight mass spectrometry was used to analyze the eluate from a high-performance liquid chromatography separation. The mass spectrum of one chromatographic peak, containing a protein of unknown molecular mass, displays MH peaks at m/z = 5 654.208, 5 277.326, 4 947.590, 4 656.613, 4 397.990, and 4 166.576. Find the average molecular mass (M) of the neutral protein and its standard deviation.
- Bradford assay was used to determine bovine serum albumin (BSA) concentrations. The method works by binding Coomassie Brilliant Blue dye to protein standards and unknown, leading to a shift in the absorbance maximum of the dye. A stock solution of BSA is available (10 mg/ml) from which the protein standards were prepared. Bradford reagent, 290 ul, was pipetted into a transparent 96-well microplate. 10 ul of the protein dilution was added followed by mixing in the wells. After 5 min of incubation at room temperature, the plate was read in spectrum mode with the absorbance spectrometer of the microplate reader. The spectrum of the dye not bound to protein was also determined. Create a standard curve or regression equation that related the protein concentration and absorbance. An unknown BSA sample was prepared the same way as the standard and the OD was 0.35. determine the original concentration of the protein solution. The measurements can be seen below. 0.0625 Protein Concentration…Inductively coupled plasma–optical emission spectrometry (ICP-OES) was used for the determination of cadmium using indium as an internal standard. A solution containing 2.15 ppm Cd2+ and 5.76 ppm In3+ gave signals of 19,291 and 37,190 counts for cadmium and indium, respectively. A 0.01652 g sample was dissolved in acid and quantitatively transferred to a 250.0 mL volumetric flask. A 1.00 mL aliquot of 264.5 ppm In3+ was added and the flask was diluted to volume with deionized water. ICP-OES analysis of the sample solution gave signals of 31,109 and 118,941 counts for cadmium and indium, respectively. What was concentration of the Cd2+ in the sample? Give answer all questions with explanationTwo compounds with partition coefficients of 15 and 18 are to be separated on a column with VSVM=0.30VSVM=0.30. Calculate the number of theoretical plates needed to produce a resolution of 1.8. Do not worry about significant figures of your final answer.
- A Dubosq colorimeter consists of a cell of fixed path length and a cell of variable path length. By adjusting the length of the latter until the transmission through the two cells is the same. the concentration of the second solution can be inferred from that of the former. Suppose that a plantdye of concentration 25 μg dm-3 is added to the fixed cell. the length of which is 1.55 em. Then a solution of the same dye. but of unknown concentration. is added to the second cel l. It is found that the same t ransmittance is obtained when the length of the second cel l is adjusted to 1.18 em. What is the concentration of the second solution?Two species A and B are known to have water/hexane partition coefficient of 5.99 and 6.16.They are separated by elution on silica gel with hexane as eluent. The ratio for the packingVS/VM =0.425a. Calculate the retention factor for each soluteb. Calculate the selectivity factorc. How many plates are needed to provide a resolution of 1.5?d. What column length should be used to provide a resolution of 1.5 if the plate height ofthe packing is 1.5 ×10-3cm?e. If the flow rate is 6.75 cm min-1, how long will it take to elute the two species? (the question wanted to be answered)4c. Calculate the values of Mn, Mw, Mz and polydispersity index for a polymer mixture having equivalent moles of two polymers with exact molecular weight being 2,000 Dalton and 10,000 Dalton.