What are the possible causes of having Too Numerous To Count (TNTC) bacterial colonies in an agar plate? What are the ways in which this can be prevented?
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What are the possible causes of having Too Numerous To Count (TNTC) bacterial colonies in an agar plate? What are the ways in which this can be prevented?
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- After streaking microbial culture on agar plates and observing colonial growth, TMTC usually happens. What are the causes of TMTC plates (plates with more than 300 colonies that cannot be counted)? What are the ways to prevent this from happening?In a bacterial culture on an agar plate, what is a zone of inhibition? An area where the growth of bacteria is increased compared to the rest of the agar plate An area where the growth of bacteria is prevented or reduced compared to the rest of the agar plate An area where the growth of bacteria is not affected compared to the rest of the agar plate An area where the the agar becomes contaminated with moldwhy is it important that a small amount of inoculum be used in preparation of an agar slant?
- Would you use an inoculating loop to transfer bacteria to an agar deep tube? Would you use an inoculating loop to transfer bacteria to an agar deep tube? Yes, as long as you insert the loop into the agar smoothly. No, a needle would be best since it will do the least amount of damage to the agar and allow it to reseal around the inserted bacteria. It depends on which bacteria you are transferring. Yes, as long as you are careful when streaking on the surface of the agar.How is air contamination prevented when an inoculating loop is used to introduce or take a bacterial sample to/from an agar plate?What is the description of the growth and growth pattern of the microorganisms in the spread plate method?Type of Growth: Surface or Sub-surface?What is the typical growth pattern of inocula?
- Abigail did not dry the top of her agar plate and stored it top up. Now she wants to count colonies but there is a smear over the entire plate. She was working with E. coli. Why does she have a smear?Describe characteristics of Streptococcus Agalactiae in the Agar: (How does colonies look like (color) and explain does it grow on that agar. (Don't have to write the incubation period) ~ Only describe how would it look like on the Agar: Blood Agar (Aerobic) MacConkey EMB PEA Mannitol Salt Agar Chocolate Agar Nutrient AgarDescribe characteristics of Streptococcus Agalactiae in the Agar: (How does colonies look like (color) and explain does it grow on that agar. Blood Agar (Aerobic) MacConkey EMB PEA Mannitol Salt Agar Chocolate Agar Nutient Agar
- What is the major difference between an enrichment culture and a selective culture? Why are microbial doubling times in nature typically longer than those obtained in the lab? Briefly describe the following mechanisms of measuring bacterial growth: Direct microscopic cell count Plate count Most probable numberYou are transferring a bacterial culture from a tube of broth to a Petri dish containing trypticase soy agar. Please list the steps that are necessary to make the transfer successfully and with minimal risk. of contamination.Why does uninoculated agar plates still has visible growth of microorganisms? Where do you think the microorganisms came from?