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- . a. What are all the transversions that can be made starting with the codon CGG?b. Which of these transversions will be missense? Canyou be sure?23. gene amplification results in larger amounts of gene products true or false8a. Given the following mutated sequence (with respect to the normal sequence), what TYPE of mutation occurred: AAACGTTAC 8b. Where did the mutation take place?
- 7a. Given the following mutated sequence (with respect to the normal sequence), what TYPE of mutation occurred: AAGCCGTTAC 7b. Where did the mutation take place?6a. Given the following mutated sequence (with respect to the normal sequence), what TYPE of mutation occurred: AAGCTTAC 6b. Where did the mutation take place?1. List four general methods for experimentally inhibiting the activity of a specific gene with citations.
- 1. Using the data available describe the phenotype that you observe when each of these three genes is knocked down using RNAi. Remember to compare the worms on control RNAi plates with worms on each of the RNAi knockdown plates. In each case state how do these phenotypes relate to the function of the gene. (gene name for F27C1.8 is dpy-5,for T19E7.2 is skn-1) 2. Describe further experiments that you could do that would confirm your findings.2. A single base addition and a single base deletion approximately 15 bases apart in the MRNA specifying the protein lysozyme for the bacterial virus T4 caused a change in the protein from its normal composition: Lys – Ser - Pro - Ser - Leu- Asn-Ala - Ala - Lys To the mutant form: Lys - Val - His- His - Leu - Met- Ala-Ala- Lys a. From the genetic code, decipher the sequence of mRNA for both original protein and double mutant. b. Which base was added? Which base was deleted? · END -1a. What is the historical evidence obtained by Pierre Chambon that led him to conclude that amino acid coding regions within eukaryotic genes must be interrupted by noncoding regions?
- 3). Consider the four mutations (i-iv) described below: i. One of the mutations causing cystic fibrosis in humans is a deletion of three nucleotides that eliminates a phenylalanine at position 508 of the CFTR protein (D508). Normally, CFTR protein is localized to the plasma membrane, where it functions as a chloride ion channel. D508 CFTR is misfolded and all of it is degraded without ever reaching the cell surface. ii. The yeast transcription factor Gal4p contains a DNA-binding domain and a transcriptional activation domain. An allele with a deletion the gene portion encoding the activation domain encodes a truncated Gal4p containing only the DNA-binding domain. It binds to Gal4p target genes at appropriate binding sites in their upstream regulatory regions, but does not activate their transcription. In cells with both wild type and mutant forms of Gal4p, the truncated Gal4p binds more efficiently to target DNA sequences than wild type. iii. Mutations in the acid maltase gene in…4. Different populations of mouse L-cells that lack cadherins were transfected with either E- cadherin or P-cadherin. If cells expressing E-cad are mixed with those expressing P-cad, they segregate into distinct balls of cells (A in figure). However, if cells expressing different levels of the same cadherin are mixed, they form a single ball, with the high expressing cells in the center B in the figure). (A) E-cadherin SORTING OUT P-cadherin (B) low P-cadherin SORTING OUT high P-cadherin Why do the cells expressing different levels of cadherin segregate as in (B) in the figure, and why not into two separate balls, or one ball with a different organization (randomly mixed, or high expressors on the outside)?2) How does the HAEIII enzyme discriminate between the C-G polymorphism in the TAS2R38 gene?