Well 1 contains 20 uL of serum. Wells 2-6 contain 90 uL of water. To do a serial dilution of 10% concentration (not volume) for each dilution, how many uL of liquid do you move between each well? Type your answer as a whole number of uL, but do not type the "uL" label.
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Well 1 contains 20 uL of serum. Wells 2-6 contain 90 uL of water. To do a serial dilution of 10% concentration (not volume) for each dilution, how many uL of liquid do you move between each well? Type your answer as a whole number of uL, but do not type the "uL" label. Hint: see end of video.
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- SERIAL DILUTION Dilution is the act of making a weaker solution from a stronger one. This is usually done by adding a water or saline, which contains none of the material being diluted. Dilution is usually expressed as one unit of the original solution to the total number of units of final solution. Serial dilution means decreasing the volume of serum progressively by maintaining a constant volume of fluid. Most commonly, serial dilutions are twofold, that is, each dilution is half as concentrated as the preceding one. The total volume in each tube is the same. Principle: Serial dilutions are a set of dilutions in which the dilution factor is exactly the same at each step. These are used to make high dilutions with a small number of test tubes and a minimal amount of diluent. This is commonly done to determine the strength or tier of a particular antibody in patient serum diagnosis of a disease state. Traditionally, serological pipettes have been used in this process, but now it is…The laboratory received a 24-hour urine collection from a 26-year old male (body surface area = 2.34m2), and the total urine collection volume measured 800 mL in 24 hours. After creatinine determinations were performed by the alkaline picrate method, interpret the result. Plasma creatinine: 1.2 mg/dL Urine creatinine: 150 mg/dL a. The creatinine clearance of the patient is above reference range. b. The creatinine clearance of the patient is below reference range. c. The creatinine clearance of the patient is within reference range. d. The creatinine clearance of the patient is borderline high.Below is an image of a dilution that was performed. The volume above each arrow indicates the volume being transferred to the next tube. The volume listed at the bottom-right of each tube indicates the volume of diluent added to that tube. .5ml 300ul 1ml stock Tube 1 Tube 2 Tube 3 3ml 4ml 8.5ml a. If the stock concentration is 50µg/ml, what is the concentration of tube 3 in ug/mL? b. What is the dilution factor of Tube 1?
- Give typing answer with explanation and conclusion Order is for 40mEq of medication. You have 20mEq in 30mL. How many mL will you administer per dose? Round your answer to the tenths place.An infusion was ordered at 1.5 mcg/kg/min for a patient weighing 180lb. The available solution strength is 400 mg in 250 mL. What is the dosage in mcg/min? Group of answer choicesTube A is a 1/5 dilution and then, using Tube A, is diluted to make a 1/10 labeled Tube B. What is the dilution to be made for each tube, is using serum and saline? What is the dilution of the solution in tube 1 and 2 .
- Complete the dilution series using a maximum amount of 20 mL of dilutent so that the dilution in the final tube is 10^-9The order is to give 45 mEq. The dosage available is 30 mEq in 15 mL. How many mL do you give?You are asked to make 1/2 dilution using 1.5mL of serum. How much dilution do you need to use?
- At the beginning of the shift, drainage bottle was marked at 975 ml. At the end of the shift ,drainage was at the 1220 ml level. The amount you record is the balance between these numbers. The amount of Ml's that you should record for your shift is.In one square of a hemacytometer you count 55 cells. What is the approximate concentration of cells per l (liter) in this sample? (note that "^" means superscript, e.g. 10^2 means 10 to the 2nd power) The options are 5.5 X 10^5 2.75 X 10^8 5.5 X 10^8 2.75 X 10^5You need to administer furosemide (Lasix) 2 mg/minute via continuous IV infusion on a controller. Pharmacy has sent a bag of Lasix 400 mg diluted in D5W 250 mL. How many milliliters per hour will you set on the controller? Fill in the blanks. Answer: ml/hr