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- https://youtu.be/w7aIxiZQ60g Multiplexing agglutination https://youtu.be/uWStmyJ5Qc0 This is the multiplexing agglutination. Lab report I don’t really know what to talk about, the data, conclusions and the purpose of this. Need help pleaseThis is homework not a test! From NTSA case study https://static.nsta.org/case_study_docs/case_studies/cystic_fibrosis.pdf Please help with questions 2, 3 and 4 of part four 2. "The successful use of gene therapy to cure SCID syndrome (2000) is hoped to be a permanent cure for those patients because a good copy of the problem gene was inserted into the patients' blood stem cells in the bone marrow (hematopoietic stem cells). Once white blood cells enter the blood stream they have a limited life span, on the order of a few week to months. The blood stem cells are the cells that create more white blood cells to replace those that are lost. If the gene was only inserted into the circulating mature white blood cells, the patient would only be temporarily cured until those cells were used up or died." The current gene therapy approaches to cure CF involve inserting a functional CFTR gene into the mature epithelial cells of the lungs. In light of the preceding paragraph, do you think that…Following is the data and notice that it is a terrible idea to culture hMSCs longer than 10 days. You’re strongly Days # cells0 50001 75002 125003 125004 218005 287006 530007 1143008 1653009 19200010 19200011 11680012 8950013 8830014 78300 Part1 You are working for a start-up that is pursuing a clinical trial. The trial involves grafting hMSCs intopatients suffering from interveterbral disc disease using a degradable polymer scaffold. You are going to 3Dprint a porous cylindrical scaffold that is 2 cm in radius and 1 cm in height (matching the dimensions of adegenerated disc). Assume a porosity of 50%. You will fill available volume of the scaffold with hMSCs at adensity of 1 million cells per cm3. Based on the data above, what starting number of cells will you use andhow long will it take you to get enough cells for the trial? Part2The trial is a failure (patients did not report any reduction in back pain). Your team wants to try againusing 85% hMSCs and 15% nucleus pulposus cells .…
- In a Cervical Cancer screening program, 500 asymptomatic women were examined using the Pap smear test. 200 of the women who truly had lesions were correctly classified; 14 with lesions were wrongly classified as negative. 253 women who had no lesions were correctly classified; 33 women with no lesions were wrongly reported as positive. (A) Summarise the information provided in an appropirate table. (B) Based on the information above, calculate the Sensitivity, Specificity, Positive and Negative Predictive Values of the Pap smear test.True or false? Codes 90476-90748 identify the vaccine product only.How reliable are Lateral flowimmunochromatographic assays when compared to molecular diagnostic tests or other serological tests? Please discuss referring to original articles example of covid 19..
- How does Complement fixation test differ from haemolytic titration assay for complement? (Answer should not be more than three lines)MCQ --Rotarix is a vaccine indicated for the prevention of Rotavirus gastroenteritis caused by G1 and non-G1 types (G3, G4, and G9). Which of the following is NOT correct regards this vaccine? following immunisation, children may become irritable and experience diarrhoea ill infants or who born with SCID should not be immunized is live-attenuated vaccine derived from the G1P[8] rotavirus strain is prepared for IV administration is given in two doses None of the abovePlasma IL-12, IL-17, IL-18 and IL-4 concentrations of 36 Systemic Lupus Erythematosus (SLE) patients were determined. Eighteen sex- and age-matched control subjects were included. The cytokine concentrations were determined by enzyme linked immunosorbent assay. The mean duration of the diagnosis of SLE at the time when patients were evaluated for this study was 5.9-6.8 years. The results are shown in Table 1. Table 1 Mean ±s.d. (pg/ml) Cytokine Patients Controls P value IL-12 166.7±84.5 93.5土39.2 <0.001 IL-17 76.5±45.7 37.6±35.3 0.002 IL-18 IL-4 368.7±199.5 27.1±15.3 141.1土47.1 17.3±7.2 <0.001 <0.05 Discuss the distribution of the cytokines shown in this study and relates them with the pathogenicity of SLE.
- 2. https://doi.org/10.1186/s12868-022-00692-1 (link to research) a) In the immunohistochemistry section of the materials and methods section the authors wrote “The number of positive cells in hotspot areas in ten high power fields (HPFs) in areas of demyelination and plaques in the brain stem were counted using the image analysis software (Lecia Application Suite Version 4.12.0, Welzlar, Germany).” Why were they looking at demyelination areas for this study? b) In the effect of mitoxantrone on histopathological changes in the brain section of the results section the authors wrote “Active plaques revealed inflammatory cellular infiltrates with abundant macrophages stuffed with myelin debris, an evidence of ongoing myelin breakdown.” What does macrophages stuffed with myelin debris have to do with the study?hi, can I please get help on a case study on nueroanatomy I have been struggling for a couple of hours now and can't seem to understand the study to answer the following questions. is there any way or format that i can get help. I would really appreciate it. thanks! 1. Based on the information in the case, what is the most likely neuroanatomic location for a single lesion that can explain all of the patient’s symptoms and signs? In your own words, explain how you arrived at that localization. 2.What are some possibilities for the nature of the lesion (e.g., stroke, tumor, trauma, etc.)? In your own words, explain your rationale for these options. 3. How does the laboratory data and neuroimaging demonstrate the actual lesion for the patient? Describe how you interpret the data in your own words. 4.How was the patient was treated, and how did they subsequently fare? Describe the treatment plan in your own words.To diagnosis, we used a procedure known as a "sandwich ELISA" (Enzyme-Linked Immunosorbent Assay). 1.In the "ELISA sandwich," what is the "meat"? 2.Both patients A and B had positive results. Why are these two patients' outcomes so different? 3.What is one potential cause of an ELISA false negative result? What is one potential cause of an ELISA false positive result? What is another example of how an ELISA assay should be used?