Vida works in the haematology laboratory of a small hospital. The laboratory’s haematology procedure manual contains instructions for staining blood smears and calls for applying the stain for 2 minutes and the buffer for 2 minutes. Vida stained three patient blood smears, but the smears appeared bluer than normal. When she examined the smears microscopically, the cells also appeared blue, making it difficult to identify them. 1. What can cause blood smears to stain too blue? 2. Discuss factors that could have affected the stain results.

Complete Textbook Of Phlebotomy
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ISBN:9781337464314
Author:Hoeltke
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Chapter7: The Challenge Ofphlebotomy
Section: Chapter Questions
Problem 5.1E
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Vida works in the haematology laboratory of a small
hospital. The laboratory’s haematology procedure manual
contains instructions for staining blood smears and calls
for applying the stain for 2 minutes and the buffer for 2
minutes. Vida stained three patient blood smears, but the
smears appeared bluer than normal. When she examined
the smears microscopically, the cells also appeared blue,
making it difficult to identify them.
1.
What can cause blood smears to stain too blue?
2.
Discuss factors that could have affected the stain results.

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