Use to answer the questions. Evidence was collected at a crime scene and sequenced. DNA samples were collected from 4 possible suspects (A.B.C.D). Based on the genetic evidence, which of the suspects is the likely guilty party? Refi Ac Why did you choose that suspect? Use the gel data to support your accusation. Cla
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- sampies B. In this picture of gel electrophoresis of DNA, which sample contains the longest/largest piece of DNA? O A. A O B. Bule 11 Making Cells – 202 Bb Module 11 Making Cells - 2021 X Bb Take Test: "Lab 11 Homework - X + A sunyocc.open.suny.edu/webapps/assessment/take/launch.jsp?course_assessment_id=_109869_1&course_id=_53817_1&c Question Completion Status: D. Organelle A. Two identical that builds copies of DNA microtubule "highways" to guide DNA B. "staple" that holds DNA copies together C. DNA that is same length and has same instructions; one from mother and one from father Click Save and Submit to save and submit. Click Save All Answers to save all answers. Save All Answers MacBook DD 00 000 F8 F6 F7 F4 F5 F2 F3 F1 * $ % & @ # 2 3 4 * CO < OThe results of gel electrophoresis of 4 different DNA samples given in the figure. 16 ul was loaded into each well (6 μL diluted DNA* + 8 μL water + 2 μL sample buffer). The ladder is in lane 5, with the size and amount (ng) in each band indicated. *1:5 dilutions of the original DNA sample were made, and the diluted samples were used for AGE. Q. These are the conclusions made about sizes and concentrations of the original (undiluted) DNA samples from lanes 1 and 2. Are these conclusions reasonable? Explain specifically, and if any of the conclusions are not reasonable, explain why not and what should we conclude instead. Sample in lane 1:size is 2.32 kb concentration of original DNA sample is 9.2 ng/ul Sample in lane 2:size is 4.36 kb concentration of original DNA samples is 100 ng/ul Q. The sample in lane 3 was expected to be about 2 kb in size. What is a possible explanation for the results observed?
- True or False: 1. The concept of DNA was discovered through a series of careful experiments. 2. The concept of DNA in its early development was initiated by Avery’s group.Can you please check my answer and make sure it is correct. Question: How can DNA evidence be used to convict or exonerate a defendant? Why is DNA evidence so powerful? Answer: DNA evidence can be used to perform DNA profiling to determine the genotype of the specific DNA sample. With just a small amount of DNA, PCR can produce billions of copies of that specific segment. The segments that are used are from non-coding regions that contain STR’s or short tandem repeats. These very short DNA sequences are repeated and are specific to individuals because we inherit them from our mother and father. Gel electrophoresis separates the PCR products based on their size and each band is compared to the allele ladder. This process helps to identify the alleles present in the original samples. DNA profiling is performed at many loci to be able to tell the genetic difference between different individuals with a lot of certainty. The DNA from the different suspects is compared to the allele…- Explain how DNA fingerprints are created and analyzed using the documents below. Describe the type of data that can be gathered from a DNA fingerprint as it relates to COVID-19. - Identify two individuals from Document A who are likely most closely related. Justify your claim with evidence from the documents. - Examine Document D. Lanes 1 and 9 include DNA fragments of known lengths and are used as reference only. Predict the type of analysis that will be conducted on this DNA profile (suspect identification, relatedness, genetic disease). Justify your prediction with evidence. (Documents A through D are related to this question)
- Questions: Answer questions 1 to 3 about this gel electrophoresis. 1. Which color represents the smallest DNA fragment? (explain) 2. If “A" is the crime scene and B to E are suspects. Do you have any match? (Why or Why not) 3. Is there a possibility that any of the suspect related to the person who committed the crime? Explain.DNA Profiles as Tools for Identification A PCR-based paternity test is conducted using STRs that consistently produce a unique DNA fragment pattern from a single chromosome. Examining the results of the following Southern blot, which male(s) can be excluded as the father of the child? Which male(s) could be the father of the child?a. DNA fingerprinting is a method of visualizing similarities and differences in the DNA sequence of individuals. Arrange the following items so that they show the DNA fingerprinting steps in proper order: - Gel electrophoresis - Stain or probe for DNA - cut DNA into fragments - Amplify DNA sample using PCR b. On the gel shown below are four DNA samples. Samples A to C are taken from tissues of landslide victims that are being identified, while sample D came from a hair sample brought by a mother looking for the remains of her son. (see img) i. If similar band patterns in a gel are created using the same restriction enzyme, what does that tell you about the DNA sequence of the samples? ii. In sample C, only two fragments were created. How many restriction sites (regions where enzymes cut) are present in sample C?
- Gel Electrophoresis Background and Protocol: Gel electrophoresis is a laboratory technique that separates molecules by size using an electric current. The test has a positive and negative side. Do you believe DNA should be loaded on the positive (red) side or the negative (black) side? Please explain why using scientific reasoning. Will larger DNA bands be closer or farther away from the well where you administered samples? Why?QUESTION: There is an RFLP pattern that belongs to a disease. I need to find an inheritance pattern and marker related to the disease..True or False. During gel electrophoresis, small DNA fragments are found towards the top of the gel True False