unfractionated Figure Q22-3 Spleen colony formation by cells enriched for stem cells and by enriched cells cells unfractionated bone marrow cells (Problem 22-9). 1 10' 10 10 10 10 10 number of cells injected number of colonies
t is possible to purify hematopoietic stem cells
using a combination of antibodies directed against cell-
surface targets. By removing cells that expressed surface
markers characteristic of specific lineages such as B cells,
granulocytes, myelomonocytic cells, and T cells, investi-
gators generated a population of cells enriched for stem
cells. They further enriched this population for putative
stem cells by positively selecting for cells that expressed
suspected stem-cell surface markers. Spleen colony for-
mation in irradiated mice by these putative stem cells and
the unfractionated bone marrow cells is shown in Figure
Q22–3. Given that only about 1 in 10 cells lodges in the
spleen, do these results support the idea that the enriched
population consists mostly of hematopoietic stem cells?
What additional information would you need to have to
feel confident that the enriched cells are true stem cells?
What proportion of bone marrow cells are hematopoietic
stem cells?
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