Principles of Instrumental Analysis
7th Edition
ISBN:9781305577213
Author:Douglas A. Skoog, F. James Holler, Stanley R. Crouch
Publisher:Douglas A. Skoog, F. James Holler, Stanley R. Crouch
Chapter13: An Introduction To Ultraviolet-visible Molecular Absorption Spectrometry
Section: Chapter Questions
Problem 13.21QAP
Related questions
Question
6
![When you use the same concentration of phosphoric acid, you get the following one:
JI150928_rac_elegy : FXUVUHPDet-4 1:1
1000
900
800-
700
600
500
400
300
200
100
8.
10
Time (min)
Which separation is better? What can cause this behaviour (if all other conditions are the same, save for the acid)?
Absorbance (mAU)
Uju E82
-2.953 min
3.986 min
5.355 min](/v2/_next/image?url=https%3A%2F%2Fcontent.bartleby.com%2Fqna-images%2Fquestion%2F101d43bb-eed2-4967-b855-cd55af99a6d9%2F47eba568-01c9-4fda-8195-fdc5e0bf9d72%2F9gbv2uj_processed.png&w=3840&q=75)
Transcribed Image Text:When you use the same concentration of phosphoric acid, you get the following one:
JI150928_rac_elegy : FXUVUHPDet-4 1:1
1000
900
800-
700
600
500
400
300
200
100
8.
10
Time (min)
Which separation is better? What can cause this behaviour (if all other conditions are the same, save for the acid)?
Absorbance (mAU)
Uju E82
-2.953 min
3.986 min
5.355 min
![3. You are using reverse phase HPLC to separate the cis and trans isomers of the following compound:
он
When you use formic acid to acidify mobile phase, you get the following chromatogram:
J150928_rac : FXUVUHPDet-4 1:1
600
550
500
450
400
350
300
250
200
150
100
50
10
12
Time (min)
Absorbance (mAU)
3,098 gimin
3,510 min
913 MIA
-4.220 min
BE min
5.540min
-6.639 min
9.331 min](/v2/_next/image?url=https%3A%2F%2Fcontent.bartleby.com%2Fqna-images%2Fquestion%2F101d43bb-eed2-4967-b855-cd55af99a6d9%2F47eba568-01c9-4fda-8195-fdc5e0bf9d72%2Fdbivwps_processed.png&w=3840&q=75)
Transcribed Image Text:3. You are using reverse phase HPLC to separate the cis and trans isomers of the following compound:
он
When you use formic acid to acidify mobile phase, you get the following chromatogram:
J150928_rac : FXUVUHPDet-4 1:1
600
550
500
450
400
350
300
250
200
150
100
50
10
12
Time (min)
Absorbance (mAU)
3,098 gimin
3,510 min
913 MIA
-4.220 min
BE min
5.540min
-6.639 min
9.331 min
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