There were 2 spots on the TLC that did not match up with any of the standards. What were the Rf values of these spots? Identify what these chemical species might be and draw their structures. Solvent was 3:1:1 n-butanol/acetic acid/water. First plate is TLC plate 1. Second plate is TLC plate 2.
There were 2 spots on the TLC that did not match up with any of the standards. What were the Rf values of these spots? Identify what these chemical species might be and draw their structures. Solvent was 3:1:1 n-butanol/acetic acid/water. First plate is TLC plate 1. Second plate is TLC plate 2.
Chapter14: Chromatography
Section: Chapter Questions
Problem 1P
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There were 2 spots on the TLC that did not match up with any of the standards. What were the Rf values of these spots? Identify what these chemical species might be and draw their structures.
Solvent was 3:1:1 n-butanol/acetic acid/water.
First plate is TLC plate 1. Second plate is TLC plate 2.
Transcribed Image Text:chromatographed material after development. Hint: Lay your TLC plate on a paper towel before
doing the spotting. This will cushion the plate and allow you to pick it up more easily.
Table 2. TLC Plate Preparation
TLC Plate I
TLC Plate II
Spot Spotting
Solution to Spot
Spot
Spotting
Solution to Spot
12
1
3 times
Solution 1 (aspartame)
Beverage A (Solution 5)
times
12
2
1 time
Solution 2 (phenylalanine)
6.
Beverage B (Solution 6)
times
12
Beverage C (Solution
7)
1 time
Solution 3 (aspartic acid)
times
12
Solution 4 (hydrolyzed
4
8.
Solution 1 (aspartame)
3 times
times
aspartame)
Once you are satisfied that you have prepared Plate II with appropriate samples, carefully place it
in the TLC chamber (remember-the level of the solvent must be below the spots on the plate)
Transcribed Image Text:INCHES
2.
LABORATORY APPARATUS. FURNITURE
SCIENTIFIC INSTRUMENTS & CHEMICALS
INCHES
2.
LABORATORY APPARATUS. FURNITURE
SCIENTIFIC INSTRUMENTS & CHEMICALS
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