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- The spectrophotometer is used to measure absorbance experimentally. Briefly, explain how this is accomplished.
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- How will you use a solution of known concentration of one of the food dyes to determine the relationship between concentration and absorbance? Be sure to explain how you determined the wavelength for your analysis.Briefly explain why it is NOT accurate to calculate the concentration of a sample whose absorbance is 0.185 using the equation y=0.0022x+0.0005 r^2=0.999A student calibrated the mini-spectrophotometer with a blank solution, and then went to check their solution. They got a fingerprint on the cuvette while filling it. Because of this, the expected reading for the absorbance would be too high which would cause the calculated value of K, to be too high
- a) A colorimeter is a type of spectrophotometer which is used to measures the light transmitted through a sample at a specific wavelength. True or False. b) As the cell path length shortens, the percent transmittance increases. True or false. c) As the absorbance value increase, the transmittance decrease. True or false. d) Ultraviolet-visible spectroscopy measures the absorption of plane-polarized light to determine the three-dimensional structure of a compound. True or false.Given the following sample concentrations and corresponding absorbances, construct a standard curve of absorbance vs. concentration and determine the molar absorptivity constant. Sample Concentration 15 μΜ 25 μΜ 40 μΜ Absorbance 0.19 0.32 0.49 55 μΜ 0.70A solution containing two different fluorescent compounds, Ben and Jerry, were analyzed for their individual concentrations in the mixture. Standards of pure Ben and pure Jerry were prepared at a concentration of 500.0 mM and were run in a UV-Vis Spectrophotometer to determine their absorption properties. Absorbance Wavelength Compound Ben 500 mM Compound Jerry 500 mM 400 nm 0.137 0.136 450 nm 0.312 0.113 500 nm 0.154 0.078 550 nm 0.076 0.079 600 nm 0.227 0.148 650 nm 0.230 0.230 700 nm 0.151 0.357 750 nm 0.157 0.246 800 nm 0.154 0.154 A standard curve of the standards was also prepared to help determine the concentration of each component in the solution. The solution produced an absorbance reading of 0.486 at the λmax of Ben, and 0.463 at the λmax of Jerry. STD CURVE BEN Λmax Ben Λmax Jerry STD CURVE JERRY Λmax Ben Λmax Jerry CONC (mM) ABS ABS CONC (mM)…
- A solution containing two different fluorescent compounds, Ben and Jerry, were analyzed for their individual concentrations in the mixture. Standards of pure Ben and pure Jerry were prepared at a concentration of 500.0 mM and were run in a UV-Vis Spectrophotometer to determine their absorption properties. Absorbance Wavelength Compound Ben 500 mM Compound Jerry 500 mM 400 nm 0.137 0.136 450 nm 0.312 0.113 500 nm 0.154 0.078 550 nm 0.076 0.079 600 nm 0.227 0.148 650 nm 0.230 0.230 700 nm 0.151 0.357 750 nm 0.157 0.246 800 nm 0.154 0.154 A standard curve of the standards was also prepared to help determine the concentration of each component in the solution. The solution produced an absorbance reading of 0.486 at the λmax of Ben, and 0.463 at the λmax of Jerry. STD CURVE BEN Λmax Ben Λmax Jerry STD CURVE JERRY Λmax Ben Λmax Jerry CONC (mM) ABS ABS CONC (mM)…A solution containing two different fluorescent compounds, Ben and Jerry, were analyzed for their individual concentrations in the mixture. Standards of pure Ben and pure Jerry were prepared at a concentration of 500.0 mM and were run in a UV-Vis Spectrophotometer to determine their absorption properties. Absorbance Wavelength Compound Ben 500 mM Compound Jerry 500 mM 400 nm 0.137 0.136 450 nm 0.312 0.113 500 nm 0.154 0.078 550 nm 0.076 0.079 600 nm 0.227 0.148 650 nm 0.230 0.230 700 nm 0.151 0.357 750 nm 0.157 0.246 800 nm 0.154 0.154 A standard curve of the standards was also prepared to help determine the concentration of each component in the solution. The solution produced an absorbance reading of 0.486 at the λmax of Ben, and 0.463 at the λmax of Jerry. STD CURVE BEN Λmax Ben Λmax Jerry STD CURVE JERRY Λmax Ben Λmax Jerry CONC (mM) ABS ABS CONC (mM)…An aqueous solution of a Ni2+ complex with a concentration of 8.61 × 10–5 M was analyzed by a UV-VIS spectrometer at 470 nm and in a 1.00-cm cell; a percent transmittance value of 78.70% was recorded. What is the absorbance of the solution? * Provide your answer with three decimal places and without units. Avoid using scientific notation.
- Briefly describe how a phase-contrast microscope work and the kind of image that itproduces. Give a specific use for this type of microscope.During your experiment, you measure the absorbance of a sample to be 1.362. You have to convert thisabsorbance to methylene blue concentration (in ppm) using the absorbance measurements of two standardsolutions of known concentration:5 ppm: Absorbance 0.55510 ppm: Absorbance 1.211What is, in ppm, the concentration of your sample? Report using 3 decimal figures. Your answer must becorrect to 0.02 ppmIndicate the relationship of proportionality that exists between: a) concentration and absorbance b) absorbance and transmittance
