The peak parameters for your internal standard, evaluated using a capillary column with a length of 46 were as follows, retention time, 18.46 min and the peak width of 0.592 min (FWHM). What is the HETP for this separation (report your value in microns)?

Appl Of Ms Excel In Analytical Chemistry
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ISBN:9781285686691
Author:Crouch
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Chapter15: Electrophoresis And Other Separation Methods
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Question 1
The peak parameters for your internal standard, evaluated using a capillary column
with a length of 46 were as follows, retention time, 18.46 min and the peak width of
0.592 min (FWHM). What is the HETP for this separation (report your value in
microns)?
Question 2
Two analytes have separation factor of 1.05 (i.e. alpha). How many plates are requires
to realize a resolution 2.79?
Question 3
The partition coefficient of a compound between two liquid phases is 4.34. If the
volume of phase 1 is 9.00 mL, what volume, in mL, of phase 2 is needed to extract
0.96 % in a single extraction?
Transcribed Image Text:Question 1 The peak parameters for your internal standard, evaluated using a capillary column with a length of 46 were as follows, retention time, 18.46 min and the peak width of 0.592 min (FWHM). What is the HETP for this separation (report your value in microns)? Question 2 Two analytes have separation factor of 1.05 (i.e. alpha). How many plates are requires to realize a resolution 2.79? Question 3 The partition coefficient of a compound between two liquid phases is 4.34. If the volume of phase 1 is 9.00 mL, what volume, in mL, of phase 2 is needed to extract 0.96 % in a single extraction?
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