The change in the amount of product B in a reaction which is catalyzed by Enzyme A is given in the table below. Determine the Vmax and Km of the enzyme in the absence and presence of C. Is C an inhibitor or an activator of enzyme A? Determine the type of inhibition or activation.

Biochemistry
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ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
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The change in the amount of product B in a reaction which is catalyzed by Enzyme A
is given in the table below. Determine the Vmax and Km of the enzyme in the absence
and presence of C. Is C an inhibitor or an activator of enzyme A? Determine the type
of inhibition or activation.
Rate of formation of Product B in the presence
of 25 mg/mL C (mM/min)
Substrate
Rate of formation of
(mM)
Product B (mM/min)
0.5
21.5
14.1
1
30.2
22.2
1.5
34.9
27.0
2.5
39.8
33.1
3.5
42.0
36.3
Transcribed Image Text:The change in the amount of product B in a reaction which is catalyzed by Enzyme A is given in the table below. Determine the Vmax and Km of the enzyme in the absence and presence of C. Is C an inhibitor or an activator of enzyme A? Determine the type of inhibition or activation. Rate of formation of Product B in the presence of 25 mg/mL C (mM/min) Substrate Rate of formation of (mM) Product B (mM/min) 0.5 21.5 14.1 1 30.2 22.2 1.5 34.9 27.0 2.5 39.8 33.1 3.5 42.0 36.3
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