TABLE Q5-1 Frequencies of mutant cells in multiple cultures (Problem 5–6) Culture (mutant cells/106 cells) Experiment 1 2 3 4 5 6 7 8 9 10 4 0 257 1 0 o 2 32 2 2 128 0 1 4 0 O 66 2 LO
TABLE Q5-1 Frequencies of mutant cells in multiple cultures (Problem 5–6) Culture (mutant cells/106 cells) Experiment 1 2 3 4 5 6 7 8 9 10 4 0 257 1 0 o 2 32 2 2 128 0 1 4 0 O 66 2 LO
Case Studies In Health Information Management
3rd Edition
ISBN:9781337676908
Author:SCHNERING
Publisher:SCHNERING
Chapter2: Information Protection: Access, Archival, Privacy, And Security
Section: Chapter Questions
Problem 2.40.9C
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Question
To determine the reproducibility of mutation fre-
quency measurements, you do the following experiment.
You inoculate each of 10 cultures with a single E. coli bac-
terium, allow the cultures to grow until each contains 106
cells, and then measure the number of cells in each culture
that carry a mutation in your gene of interest. You were so
surprised by the initial results that you repeated the experi-
ment to confirm them. Both sets of results display the same
extreme variability, as shown in Table Q5–1. Assuming that
the rate of mutation is constant, why do you suppose there
is so much variation in the frequencies of mutant cells in
different cultures?
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