Table 2-Determination of the specificity of catechol oxidase enzyme Hydroquinone dH₂O (mL) (mL) Tube # 2b 3b Potato extract (mL) 1 1 5 5 Catechol (mL) 2 0 0 Q9) Which substrate does this enzyme catalyze best / fastest? 2 Absorbance 0 mins How does your 0.049 Based on the data from Table 2 answer these questions: Q8) Is the enzyme specific for catechol only (i.e. doesn't catalyze hydroquinone)? Note - if absorbance is negative or near zero it means no product is formed. Answer (circle one): YES NO 0.000 data support this?

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ISBN:9781305577206
Author:Reginald H. Garrett, Charles M. Grisham
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Chapter18: Glycolysis
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Table 2-Determination of the specificity of catechol oxidase enzyme
Hydroquinone
dH₂O
(mL)
(mL)
Tube #
2b
3b
Potato
extract
(mL)
1
1
5
5
Catechol
(mL)
2
0
0
Q9) Which substrate does this enzyme catalyze best / fastest?
2
How does your
Absorbance
0 mins
Based on the data from Table 2 answer these questions:
Q8) Is the enzyme specific for catechol only (i.e. doesn't catalyze hydroquinone)? Note - if absorbance is
negative or near zero it means no product is formed. Answer (circle one): YES NO
4. Effect of Temperature
0.049
0.000
data support this?
Temperate indicates the amount of energy in an object. In a liquid, when temperature decreases, the molecules
inside the liquid have lower energy levels and therefore move slowly. In a solution containing enzyme and
substrate molecules, this means that there is a lower chance of substrate bumping into or encountering enzymes
because all molecules in that cold solution are moving slowly. As temperature increases, the energy of the
molecules in the solution increases. This causes them to move faster. In a solution containing enzymes and
substrate this means that there is a higher chance of substrate encountering enzymes because all molecules in that
warm solution are moving faster.
Generally, rate of enzyme catalyzed reactions doubles with every 10°C increase in temperature. At some point
the enzymes will encounter the optimum temperature where the speed at which substrate is encountering
enzymes is at its fastest. When temperature rises above this optimum temperature, the high energy state of the
molecules in this hotter solution can cause problems. This is because most enzyme are proteins composed of
polypeptide chains. As temperature increases beyond the optimum level, the high energy state of the molecules
in the enzyme start to strain the bonds (e.g. hydrogen bonds, sulfide bonds, Van der Waal bonds) and affect the
integrity of the enzyme's secondary, tertiary and quaternary structure. These are bonds that maintain the 3-
dimensional structure of enzymes and are not as strong as covalent bonds found between amino acids in the
enzyme's polypeptide chains. Initially the 3-dimensional shape of the active site becomes slightly changed so that
the enzyme does not catalyze its substrate very well (i.e. rate of reaction slows). As temperature gets hotter beyond
the optimum, the 3-dimensional shape of the enzyme becomes permanently changed (denatured). Denaturation
is a permanent change that results in permanent loss of enzymatic function and loss of its 3-dimensional shape.
92
Transcribed Image Text:Table 2-Determination of the specificity of catechol oxidase enzyme Hydroquinone dH₂O (mL) (mL) Tube # 2b 3b Potato extract (mL) 1 1 5 5 Catechol (mL) 2 0 0 Q9) Which substrate does this enzyme catalyze best / fastest? 2 How does your Absorbance 0 mins Based on the data from Table 2 answer these questions: Q8) Is the enzyme specific for catechol only (i.e. doesn't catalyze hydroquinone)? Note - if absorbance is negative or near zero it means no product is formed. Answer (circle one): YES NO 4. Effect of Temperature 0.049 0.000 data support this? Temperate indicates the amount of energy in an object. In a liquid, when temperature decreases, the molecules inside the liquid have lower energy levels and therefore move slowly. In a solution containing enzyme and substrate molecules, this means that there is a lower chance of substrate bumping into or encountering enzymes because all molecules in that cold solution are moving slowly. As temperature increases, the energy of the molecules in the solution increases. This causes them to move faster. In a solution containing enzymes and substrate this means that there is a higher chance of substrate encountering enzymes because all molecules in that warm solution are moving faster. Generally, rate of enzyme catalyzed reactions doubles with every 10°C increase in temperature. At some point the enzymes will encounter the optimum temperature where the speed at which substrate is encountering enzymes is at its fastest. When temperature rises above this optimum temperature, the high energy state of the molecules in this hotter solution can cause problems. This is because most enzyme are proteins composed of polypeptide chains. As temperature increases beyond the optimum level, the high energy state of the molecules in the enzyme start to strain the bonds (e.g. hydrogen bonds, sulfide bonds, Van der Waal bonds) and affect the integrity of the enzyme's secondary, tertiary and quaternary structure. These are bonds that maintain the 3- dimensional structure of enzymes and are not as strong as covalent bonds found between amino acids in the enzyme's polypeptide chains. Initially the 3-dimensional shape of the active site becomes slightly changed so that the enzyme does not catalyze its substrate very well (i.e. rate of reaction slows). As temperature gets hotter beyond the optimum, the 3-dimensional shape of the enzyme becomes permanently changed (denatured). Denaturation is a permanent change that results in permanent loss of enzymatic function and loss of its 3-dimensional shape. 92
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