Table 1. Sample Set A (PNPP concentration = 0.05mM) Final [Pi] Vol of 1mM P (μM) (HL) [PNPP] (mM) Vol of 0.5mM PNPP Vol of 0.2M Tris-HCI (μL) (μL) Vol of enzyme Total Vol (μL) (μL) 0 0.05 100 1500 3.33 0.05 100 1500 20 0.05 100 1500 50 0.05 100 1500 100 0.05 100 1500

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Table 1. Sample Set A (PNPP concentration = 0.05mM)
Final [Pi] Vol of 1mM P
(μM)
(HL)
[PNPP]
(mM)
Vol of 0.5mM PNPP Vol of 0.2M Tris-HCI
(μL)
(μL)
Vol of enzyme Total Vol
(μL)
(μL)
0
0.05
100
1500
3.33
0.05
100
1500
20
0.05
100
1500
50
0.05
100
1500
100
0.05
100
1500
Transcribed Image Text:Table 1. Sample Set A (PNPP concentration = 0.05mM) Final [Pi] Vol of 1mM P (μM) (HL) [PNPP] (mM) Vol of 0.5mM PNPP Vol of 0.2M Tris-HCI (μL) (μL) Vol of enzyme Total Vol (μL) (μL) 0 0.05 100 1500 3.33 0.05 100 1500 20 0.05 100 1500 50 0.05 100 1500 100 0.05 100 1500
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