Suppose that there is a protein consisting of two polypeptide chains with the given sequences in the picture. Answer the following questions. a. What digestion or cleavage agent(s) should a biochemist use to determine the sequence of subunit α? Explain why. b. What digestion or cleavage agent(s) should a biochemist use to determine the sequence of subunit β? Explain why. c. Identify the resulting peptide fragment(s) of subunit α. Identify the resulting peptide fragment(s) of subunit β.

Understanding Health Insurance: A Guide to Billing and Reimbursement
14th Edition
ISBN:9781337679480
Author:GREEN
Publisher:GREEN
Chapter6: Icd-10-cm Coding
Section: Chapter Questions
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Suppose that there is a protein consisting of two polypeptide chains with the given sequences in the picture.

Answer the following questions.

a. What digestion or cleavage agent(s) should a biochemist use to determine the sequence of subunit α? Explain why.

b. What digestion or cleavage agent(s) should a biochemist use to determine the sequence of subunit β? Explain why.

c. Identify the resulting peptide fragment(s) of subunit α. Identify the resulting peptide fragment(s) of subunit β.

 

Subunit a: L-A-D-C-S-T-C-W-A-S-A-R-A-C-A-K-A-N-C-E-I-A-M
Subunit B: G-P-C-W-I-A-R-S-C-T-E-T-W-C-Y-V-V-M-L-C-K-A-D
Transcribed Image Text:Subunit a: L-A-D-C-S-T-C-W-A-S-A-R-A-C-A-K-A-N-C-E-I-A-M Subunit B: G-P-C-W-I-A-R-S-C-T-E-T-W-C-Y-V-V-M-L-C-K-A-D
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