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- Q2) Draw the standard curve for bovine serum albumin protein (BSA): First, prepare the following solutions: 1. Stock solution of BSA with 2 mg/ml concentration. 2.7 dilutions of BSA as shown in the table. Second, measure the absorbance for each tube at 280 nm. Finally, draw the standard curve for BSA. Reagents Blank Tube Tube 2 3 Tube Tube Tube Tube Tube 1 4 5 6 7 Stock solution of BSA= 2 mg/ml Distilled water Final 0.5 1 1.5 2.5 3 3.5 4.5 3.5 3 2.5 1.5 concentration (mg/ml) The 0.2 0.4 0.6 0.8 1.0 1.2 1.4 absorbance 0.086 0.422 0.665 0.727 0.858 1 1.2 at 280 nm Draw the standard curve with line of best fit. 2. 2. 4.13) Q13) The relative volume of red blood cells can be known by measuring the hematocrit (the ratio between the volume occupied by red blood cells to the total volume of blood). If the sample shown in the figure is obtained in a laboratory, what is the expected viscosity of the sample of blood? Note that represents the viscosity of blood plasma nserum 0% 100 90 80 70 60 50 40 30 20 10 0 2.17 nserum 2.6 nserum 45 nserum 55 nserum % 100 90 80 70 60 50 40 30 1016. Your Excel data graph for Serial Dilution lab:** 16a. Include a clear and labeled graph of DF (x axis) versus Absorbance (y axis). See box above for expectations and review video tutorial of this Excel work. Do not graph two of your data points: your blank or your Cuvette #1 (DF=1). 1 Q A Multiple Styles W S X 3 E D $ 4 To C R F % 5 I T V U ^ 6 G Y 7 H U 8 J BN S ( 9 K M X
- Use the following information collected from a SIZE-EXCLUSION CHROMATOGRAPHY experiment to answer the questions below. MW Standards Molecular Weight (kDa) Elution Volume (mL) blue dextran 1000 85 myoglobin 17.2 200 TIM 53.3 178 hemoglobin 62.2 156 IgG 140 122 ATCase 307.9 94 Based on the notes provided to you, which Sephadex gel filtration media would be ideal to use with the given standards? and whyA.How much of 10,000x SYBR safe would you add to 50 ml to make a final concentration of 1x? B.How will you set up the serial dilution? How many tubes do you need? What is the concentration in each? How much LB will you add to each tube? What volume of cells will you add?Q1.5 ... If the equation produces from data is y = 0.300x-0.857 and the absorbance for the unknown is 0.429. What would be the concentration of the unknown. Give the answer with 3 decimal places. Type your numeric answer and submit Unanswered a Save Q1.9 Fill in the Blanks Type your answers in all of the blanks and submit The microplate reader is used to take measurements of Type your answer here and Type your answer here
- AE, 62, Male Wt: 75 kg, Ht: 150 cm Orders: Penicillin G Potassium 10,000,000 Units Sterile water for injection qs Make a 250,000 U/mL solution. The package insert states If Penicillin G Potassium 10,000,000 Units is reconstituted with 44 mL sterile water, a solution of 200,000 U per mL will be obtained. What volume of sterile water would you use to reconstitute the penicillin G potassium in order to make the ordered solution?Time CFU at 48 hours Data Table 3 Plate description Dilution: 10-1 Incubation time: 24 Dilution: 10-2 Incubation time: 24 Dilution: 10-3 Incubation time: 24 Results: Dilution: 10-4 Incubation time: 24 Dilution: 10-5 Incubation time: 24 Dilution: 10-6 Incubation time: 24 10-1 TNTC Average CFU/mL: How do you calculate CFU/ml (what is the formula)? 10-2 TNTC TNTC Volume Number plated of CFU (ml) TNTC TNTC 250 185 TNTC TFTC 10-3 0.1 0.1 0.1 0.1 0.1 10-4 0.1 250 Dilution factor 10-5 185 10-6 TFTC CFU/mLA. a pre-weighted dialysis bag which contained a solution of 10% glucose was placed in a beaker which contained 20% glucose. After one hour, the bag was weighted again. Calculate the % mass change of the dialysis bag from the following information: Mass of bag before the experiment: 15.3g Mass of bag after the experiment: 12.7% B. was the beaker solution hypertonic, hypotonic, or isotonic?
- (e) Complete the following table: Number printed on the micropipette's end (its plunger) 20 20 200 1000 1000 Number showing in the micropipette's display window OTOHOSHOSHOOO 0 7 0 1 0 5 1 0 5 1 0 0 0 2 1 Volume that the micropipette is set to draw up (include units in your answer) 6) Before using a micropipette, you have to first place a 7) Shown below are the steps required to transfer fluid from one tube to another using a micropipette. Write numbers in the blanks to indicate the correct order in which the steps should be performed. Slowly release the micropipette's plunger. Place the micropipette tip into the tube containing fluid you wish to draw up. Push down on the micropipette plunger until it reaches the second stop. Place the micropipette tip into the tube that you wish to expel fluid into. Push down on the micropipette plunger until it reaches the first stop. 8) Suppose that you're getting ready to draw fluid into a micropipette. To do so, you push the micropipette's plunger…The figures below are the results of flow cytometer analysis of a drugs (camptotechin) effect on cells? From what you learned in class about flowcytometry, briefly state what the drug is doing to the cells: HL60 Untreated Camptothecin (45um, 2h) Camptothecin (45um, 4h) 3.36 10 PI (FL2)Q7: what do the following acronyms mean? :1- HEPA 2-BMS