Sonicating a suspension of mitochondria produces submitochondrial particles derived from the inner mitochondrial membrane. These membranous vesicles seal inside out, so that the intermembrane space of the mitochondria becomes the lumen of the submitochondrial particle. Explain (or diagram) the process of electron transfer and oxidative phosphorylation in these particles. Assuming all the substrates for oxidative phosphorylation are present in excess, does ATP synthesis increase or decrease with an increase in the pH of the fluid in which the submitochondrial particles are suspended? Why?
Sonicating a suspension of mitochondria produces submitochondrial particles derived from the inner
mitochondrial membrane. These membranous vesicles seal inside out, so that the intermembrane space of the
mitochondria becomes the lumen of the submitochondrial particle. Explain (or diagram) the process of electron
transfer and oxidative phosphorylation in these particles. Assuming all the substrates for oxidative
phosphorylation are present in excess, does ATP synthesis increase or decrease with an increase in the pH of
the fluid in which the submitochondrial particles are suspended? Why?
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Using a generalized terminology (i.e. neither CIII nor CIV specific), explain how complex III and IV act as electron/proton charge separation device (not a proton pump) in mitochondria bioenergetics to create electron potential through the iner mitochondria membrane
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