Show where trypsin and chymotrypsin would cleave the following peptide. Tyr-Ile-Gln-Arg-Leu-Gly-Phe-Lys-Asn-Trp-Phe-Gly-Ala-Lys-Gly-Gln-Gln B. After treatment with peroxyformic acid, the peptide hormone vasopressin is partially hydrolyzed. The following fragments are recovered. Propose a primary structure for vasopressin. Phe-Gln-Asn Pro-Arg-Gly • NH2 Cys-Tyr-Phe Asn-Cys-Pro-Arg Tyr-Phe-Gln-Asn C. Consider the following peptide: Gly-Ile-Glu-Trp-Thr-Pro-Tyr-Gln-Phe-Arg-Lys What amino acids and peptides are produced when the above peptide is treated with each of the following reagents? 1. Carboxypeptidase 2. Chymotrypsin 3. Trypsin 4. DNFB D. From the analytical results, deduce the primary structure of a peptide isolated from the Atlantian orchid that contains 14 amino acids. Complete hydrolysis produces the following amino acids: Gly (3), Leu (3), Glu (2), Pro, Met, Lys (2), Thr, Phe. Treatment with carboxypeptidase releases glycine. Treatment with DNFB releases DNP- glycine. Treatment with a nonspecific proteolytic enzyme produces the following fragments: Gly-Leu-Glu, Gly-Pro-Met-Lys, Lys-Glu, Thr-Phe-Leu-Leu-Gly, Lys-Glu-Thr-Phe-Leu, Leu-Leu-Gly Glu-Thr-Phe, Glu-Gly-Pro, Pro-Met-Lys-Lys, Gly-Leu Hydrolysis of β-endorphin (a peptide containing 31 amino acid residues) produces the following amino acids: Tyr (1), Gly (3), Phe (2), Met, Thr (3), Ser (2), Lys (5), Gln (2), Pro, Leu (2), Val (2), Asn (2), Ala (2), Ile, His, and Glu Treatment with carboxypeptidase liberates Gln. Treatment with DNFB liberates DNP-Tyr. Treatment with trypsin produces the following peptides: Lys, Gly-Gln, Asn-Ala-Ile-Val-Lys, Tyr-Gly-Gly-Phe-Met-Thr-Ser-Glu-Lys, Asn-Ala-His-Lys Ser-Gln-Thr-Pro-Leu-Val-Thr-Leu-Phe-Lys Treatment with chymotrypsin produces the following peptides: Lys-Asn-Ala-Ile-Val-Lys-Asn-Ala-His-Lys-Lys-Gly-Gln, Tyr-Gly-Gly-Phe Met-Thr-Ser-Glu-Lys-Ser-Gln-Thr-Pro-Leu-Val-Thr-Leu-Phe What is the primary sequence of β-endorphin?
Show where trypsin and chymotrypsin would cleave the following peptide. Tyr-Ile-Gln-Arg-Leu-Gly-Phe-Lys-Asn-Trp-Phe-Gly-Ala-Lys-Gly-Gln-Gln
B. After treatment with peroxyformic acid, the peptide hormone vasopressin is partially hydrolyzed. The following fragments are recovered. Propose a primary structure for vasopressin.
Phe-Gln-Asn Pro-Arg-Gly • NH2 Cys-Tyr-Phe Asn-Cys-Pro-Arg Tyr-Phe-Gln-Asn
C. Consider the following peptide: Gly-Ile-Glu-Trp-Thr-Pro-Tyr-Gln-Phe-Arg-Lys
What amino acids and peptides are produced when the above peptide is treated with each of the following reagents?
1. Carboxypeptidase
2. Chymotrypsin
3. Trypsin 4. DNFB
D. From the analytical results, deduce the primary structure of a peptide isolated from the Atlantian orchid that contains 14 amino acids.
Complete hydrolysis produces the following amino acids: Gly (3), Leu (3), Glu (2), Pro, Met, Lys (2), Thr, Phe. Treatment with carboxypeptidase releases glycine. Treatment with DNFB releases DNP- glycine. Treatment with a nonspecific proteolytic enzyme produces the following fragments:
Gly-Leu-Glu, Gly-Pro-Met-Lys, Lys-Glu, Thr-Phe-Leu-Leu-Gly, Lys-Glu-Thr-Phe-Leu, Leu-Leu-Gly Glu-Thr-Phe, Glu-Gly-Pro, Pro-Met-Lys-Lys, Gly-Leu
Hydrolysis of β-endorphin (a peptide containing 31 amino acid residues) produces the following amino acids:
Tyr (1), Gly (3), Phe (2), Met, Thr (3), Ser (2), Lys (5), Gln (2), Pro, Leu (2), Val (2), Asn (2), Ala (2), Ile, His, and Glu
Treatment with carboxypeptidase liberates Gln. Treatment with DNFB liberates DNP-Tyr. Treatment with trypsin produces the following peptides:
Lys, Gly-Gln, Asn-Ala-Ile-Val-Lys, Tyr-Gly-Gly-Phe-Met-Thr-Ser-Glu-Lys, Asn-Ala-His-Lys Ser-Gln-Thr-Pro-Leu-Val-Thr-Leu-Phe-Lys
Treatment with chymotrypsin produces the following peptides:
Lys-Asn-Ala-Ile-Val-Lys-Asn-Ala-His-Lys-Lys-Gly-Gln, Tyr-Gly-Gly-Phe Met-Thr-Ser-Glu-Lys-Ser-Gln-Thr-Pro-Leu-Val-Thr-Leu-Phe
What is the primary sequence of β-endorphin?
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