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Biomedical Instrumentation Systems

Biomedical Instrumentation Systems

1st Edition

ISBN:9781133478294

Author:Chatterjee

Publisher:Chatterjee

Chapter5: Biomedical Electronics: Digital

Section: Chapter Questions

Problem 3P

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Question

serotonin [µM]
0.025
transport rate A [dpm/min]
300.0
transport rate B [dpm/min]
10
0.08
920.0
30.0
0.25
2500.0
5400.0
100.0
0.7
350.0
2
8800.0
1100.0
9100.0
3000.0
14. Compare the plots of the transport rates for both conditions. What is most likely
mechanism of transport for serotonin into these cells? Explain your interpretation.
15. Assume you repeat the experiment under the same conditions as in A, but in the presence
of ouabain. What would be the effect on the transport rate for serotonin in this case? Explain.

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serotonin [µM] 0.025 transport rate A [dpm/min] 300.0 transport rate B [dpm/min] 10 0.08 920.0 30.0 0.25 0.7 2500.0 100.0 5400.0 350.0 8800.0 9100.0 2 1100.0 6 3000.0 12. What transport rates for each condition would you expect if the experiment was repeated using 12µM ['H] serotonin? Add your estimate into the table above. 13. Examine the plot obtained for condition A; what can you conclude with regards to whether or not the transport of serotonin into these cells requires a protein? Explain your conclusion.
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